Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.
Department of Pediatric Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Life Sci. 2019 Jun 15;227:51-57. doi: 10.1016/j.lfs.2019.04.043. Epub 2019 Apr 17.
Increasing evidence has shown the diagnostic value of miR-155 in organ transplantation. The dysregulation of miR-155 is reported to be associated with development of acute or chronic complications in solid organ transplant recipients. Here, we summarized related evidence to explore the correlation between the dysregulation of miR-155 and various allograft dysfunction in transplant recipients, and verified the dynamic change of miR-155 level in acute rejection (AR) using a rat renal transplantation model.
Eligible studies were retrieved from PubMed, Embase, and Cochrane Library databases. A meta-analysis method was performed to evaluate the diagnostic value of miR-155 in transplant recipients. Furthermore, the F344-Lewis rat renal transplantation model was established to validate the dynamic change of miR-155 expression during AR.
A total of 275 transplant patients, including renal, heart, and lung transplantation from 6 studies were analysed. The pooled SEN of miR-155 was 0.87 (95% CI, 0.78-0.93), the pooled SPE was 0.76 (95% CI, 0.63-0.85), the pooled PLR was 3.6 (95% CI, 2.2-5.8), the pooled NLR was 0.17 (95% CI, 0.09-0.31), the pooled DOR was 17.31 (95% CI, 7.20-41.65) and pooled AUC was 0.89 (95% CI, 0.86-0.92). The rat renal transplantation model (n = 24) and control model (n = 15) were successfully established. Expression of miR-155 in plasma was significantly increased in 7 d and 9 d post-transplantation compared to the control group (P < 0.05), and was consistent with the dynamic change of AR degree.
miR-155 is a potential biomarker for monitoring the abnormal allograft status in solid organ transplantation.
越来越多的证据表明 miR-155 在器官移植中的诊断价值。据报道，miR-155 的失调与实体器官移植受者急性或慢性并发症的发展有关。在这里，我们总结了相关证据，探讨了 miR-155 失调与移植受者各种同种异体移植物功能障碍之间的相关性，并通过大鼠肾移植模型验证了 miR-155 水平在急性排斥反应 (AR) 中的动态变化。
从 PubMed、Embase 和 Cochrane Library 数据库中检索符合条件的研究。采用荟萃分析方法评估 miR-155 在移植受者中的诊断价值。此外，建立了 F344-Lewis 大鼠肾移植模型，以验证 AR 期间 miR-155 表达的动态变化。
共分析了 6 项研究中的 275 例肾、心和肺移植患者。miR-155 的汇总 SEN 为 0.87（95%CI，0.78-0.93），汇总 SPE 为 0.76（95%CI，0.63-0.85），汇总 PLR 为 3.6（95%CI，2.2-5.8），汇总 NLR 为 0.17（95%CI，0.09-0.31），汇总 DOR 为 17.31（95%CI，7.20-41.65），汇总 AUC 为 0.89（95%CI，0.86-0.92）。成功建立了大鼠肾移植模型（n=24）和对照组模型（n=15）。与对照组相比，移植后 7d 和 9d 血浆中 miR-155 的表达显著增加（P<0.05），与 AR 程度的动态变化一致。
miR-155 是监测实体器官移植中同种异体移植物异常状态的潜在生物标志物。
