Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, USA.
Department of Mechanical and Aerospace Engineering, The George Washington University, Washington, DC, USA; Department of Biomedical Engineering, The George Washington University, Washington, DC, USA.
Nanomedicine. 2019 Jul;19:58-70. doi: 10.1016/j.nano.2019.04.002. Epub 2019 Apr 18.
Osteochondral defects resulting from trauma and/or pathologic disorders are critical clinical problems. The current approaches still do not yield satisfactory due to insufficient donor sources and potential immunological rejection of implanted tissues. 3D printing technology has shown great promise for fabricating customizable, biomimetic tissue matrices. The purpose of the present study is to investigate 3D printed scaffolds with biomimetic, biphasic structure for osteochondral regeneration. For this purpose, nano-hydroxyapatite and transforming growth factor beta 1 nanoparticles were synthesized and distributed separately into the lower and upper layers of the biphasic scaffold, which was fabricated using 3D stereolithography printer. Our results showed that this scaffold design successfully promoted osteogenic and chondrogenic differentiation of human bone marrow mesenchymal stem cells, as well as enhanced gene expression associated with both osteogenesis and chondrogenesis alike. The finding demonstrated that 3D printed osteochondral scaffolds with biomimetic, biphasic structure are excellent candidates for osteochondral repair and regeneration.
创伤和/或病理紊乱导致的软骨下骨缺损是临床上的重大难题。目前的方法仍然不能令人满意，因为供体来源不足和植入组织的潜在免疫排斥。3D 打印技术在制造定制化、仿生组织基质方面显示出巨大的潜力。本研究旨在探索用于软骨下骨再生的具有仿生双相结构的 3D 打印支架。为此，合成了纳米羟基磷灰石和转化生长因子-β1 纳米颗粒，并分别分布在双相支架的上下层中，该支架是使用 3D 立体光刻打印机制造的。我们的结果表明，这种支架设计成功地促进了人骨髓间充质干细胞的成骨和成软骨分化，并增强了与成骨和成软骨相关的基因表达。研究结果表明，具有仿生双相结构的 3D 打印软骨下骨支架是软骨下骨修复和再生的理想候选材料。
