Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore.
Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, 117585, Singapore; NUS Environmental Research Institute, National University of Singapore, 1 Create Way, Create Tower #15-02, Singapore 138602, Singapore.
Bioresour Technol. 2019 Aug;285:121333. doi: 10.1016/j.biortech.2019.121333. Epub 2019 Apr 13.
A three-stage anaerobic digester setup was configured and evaluated for enhanced methane production during co-digestion of food waste and waste activated sludge and the corresponding bacterial and methanogen communities were characterized. Results showed that the average methane yield (0.496 L/gVS) in the three-stage digester was 13-52% higher than that of one- and two-stage digesters. Compared to controls, an increase of 12-47% in volatile solids reduction was achieved in the three-stage digester (69.3 ± 6.7%). Bacterial phyla Proteobacteria, Firmicutes and Bacteroidetes dominated in one-, two- and three-stage digester while genera Pseudomonas, Tissierella, and Petrimonas were selectively enriched in the three-stage digester due to functional segregation. Taxonomic analysis identified 8 dominant methanogen genera, of which Methanosarcina, Methanosaeta, Methanobacterium and Methanolinea collectively accounted for 80%. With increasing OLR and digester stage number, the dominant methanogenic pathway shifted from hydrogenotrophic pattern to acetoclastic pattern and reached a final synergy of these two.
构建并评估了一个三段式厌氧消化器，以提高在食物垃圾和废活性污泥共消化过程中的甲烷产量，并对相应的细菌和产甲烷菌群进行了特征分析。结果表明，三段式消化器的平均甲烷产量（0.496 L/gVS）比一、二段式消化器高 13-52%。与对照相比，三段式消化器的挥发性固体减少率提高了 12-47%（69.3±6.7%）。在一、二、三段式消化器中，细菌门 Proteobacteria、Firmicutes 和 Bacteroidetes 占主导地位，而属 Pseudomonas、Tissierella 和 Petrimonas 由于功能分离而在三段式消化器中选择性富集。分类分析确定了 8 个主要的产甲烷菌属，其中 Methanosarcina、Methanosaeta、Methanobacterium 和 Methanolinea 共占 80%。随着 OLR 和消化器级数的增加，主要的产甲烷途径从氢营养型模式转变为乙酸营养型模式，并最终达到这两种模式的协同作用。
