School of Traditional Chinese Materia Medica, Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning Province, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, People's Republic of China.
Bioorg Chem. 2019 Jul;88:102926. doi: 10.1016/j.bioorg.2019.102926. Epub 2019 Apr 15.
A pair of new cycloneolignan enantiomers (1a and 1b) were isolated from the leaves of Isatis indigotica Fortune. Their structures were elucidated by extensive spectroscopic data analysis, including 1D and 2D NMR, HRESIMS, MS/MS analysis, together with theoretical electronic circular dichroism (ECD) calculations. Compounds 1a and 1b were then evaluated for their neuroprotective effects against MPP-induced SH-SY5Y cell injury. As a result, compounds 1a (77.64%) and 1b (78.62%) exhibited moderate neuroprotective activity at the concentration of 12.5 µM compared with that of MPP treated group (62.00% at 1 mM) by MTT assay. Furthermore, Annexin V-FITC/PI analysis showed that apoptosis ratios of 1a and 1b were reduced to 10.99% and 9.31%, respectively.
从菘蓝叶中分离得到一对新的环二氢菲类对映异构体（1a 和 1b）。通过广泛的光谱数据分析，包括 1D 和 2D NMR、HRESIMS、MS/MS 分析以及理论电子圆二色性（ECD）计算，确定了它们的结构。然后评估了化合物 1a 和 1b 对 MPP 诱导的 SH-SY5Y 细胞损伤的神经保护作用。结果表明，与 MPP 处理组（1mM 时为 62.00%）相比，化合物 1a（77.64%）和 1b（78.62%）在 12.5µM 浓度下通过 MTT 测定法显示出中等的神经保护活性。此外，Annexin V-FITC/PI 分析表明，1a 和 1b 的凋亡率分别降低至 10.99%和 9.31%。
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