School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Institute of TCM International Standardization of Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Molecules. 2019 Aug 29;24(17):3140. doi: 10.3390/molecules24173140.
Five pairs of alkaloid enantiomers (-) were obtained from () roots. Among them, and were determined as three pairs of new alkaloid enantiomers. Their structures were elucidated by physicochemical properties and spectroscopic methods. The absolute configurations were deduced by comparison of their experimental circular dichroism (CD) and calculated electronic circular dichroism (ECD) spectra, as well as by single-crystal X-ray crystallography using anomalous scattering of Cu K radiation. Alkaloids and possess an unpresented carbon skeleton and their putative biosynthetic pathways are discussed. Moreover, all of the alkaloids were tested for their nitric oxide (NO) inhibitory effects in RAW 264.7 cells, and and showed inhibitory effects with IC values of 76.97 μM and 65.88 μM, respectively.
从 () 根中获得了五对生物碱对映异构体（-）。其中， 和 被确定为三对对映新生物碱。通过理化性质和光谱方法阐明了它们的结构。通过比较它们的实验圆二色性（CD）和计算电子圆二色性（ECD）光谱以及使用 Cu K 辐射的异常散射的单晶 X 射线晶体学，推断了它们的绝对构型。生物碱 和 具有未呈现的碳骨架，讨论了它们的假定生物合成途径。此外，所有生物碱都在 RAW 264.7 细胞中测试了它们对一氧化氮（NO）的抑制作用， 和 显示出分别具有 76.97 μM 和 65.88 μM 的抑制作用。
