从中国安徽省采集的菘蓝叶中具有神经保护作用的生物碱。
Alkaloids with neuroprotective effects from the leaves of Isatis indigotica collected in the Anhui Province, China.
作者信息
Liu Si-Fan, Zhang Ying-Ying, Zhou Le, Lin Bin, Huang Xiao-Xiao, Wang Xiao-Bo, Song Shao-Jiang
机构信息
School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China; Chinese People's Liberation Army 210 Hospital, Dalian 116021, People's Republic of China.
School of Traditional Chinese Materia Medica, Key Laboratory of Structure-Based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China.
出版信息
Phytochemistry. 2018 May;149:132-139. doi: 10.1016/j.phytochem.2018.02.016. Epub 2018 Feb 28.
Six undescribed alkaloids, indiforine A-F, together with four known ones, were isolated from the leaves of Isatis indigotica Fortune. Their structures were elucidated on the basis of extensive spectroscopic analyses. The absolute configurations of indiforine A and B were determined by comparison of the experimental and calculated electronic circular dichroism spectra, as well as experimental and calculated optical rotations. The isolated alkaloids were evaluated for their neuroprotective activities against HO-induced cell injury in human neuroblastoma SH-SY5Y cells. The results showed that in HO-induced SH-SY5Y cell injury models, indiforine A and B exhibited potent neuroprotective activities. Further investigation of the most potent indiforine A by Hoechst 33258 staining and Annexin V/PI analysis demonstrated that it could protect SH-SY5Y cells from oxidative damage through inhibiting cell apoptosis.
从菘蓝叶中分离得到6个未描述的生物碱，靛蓝碱A - F，以及4个已知生物碱。通过广泛的光谱分析阐明了它们的结构。通过比较实验和计算的电子圆二色光谱以及实验和计算的旋光度，确定了靛蓝碱A和B的绝对构型。评估了分离得到的生物碱对人神经母细胞瘤SH - SY5Y细胞中HO诱导的细胞损伤的神经保护活性。结果表明，在HO诱导的SH - SY5Y细胞损伤模型中，靛蓝碱A和B表现出较强的神经保护活性。通过Hoechst 33258染色和Annexin V/PI分析对活性最强的靛蓝碱A进行进一步研究表明，它可以通过抑制细胞凋亡保护SH - SY5Y细胞免受氧化损伤。
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