School of Chemistry, SunYat-senUniversity, Guangzhou, 510275, China.
School of Chemistry, SunYat-senUniversity, Guangzhou, 510275, China.
Anal Chim Acta. 2019 Aug 13;1065:29-39. doi: 10.1016/j.aca.2019.03.031. Epub 2019 Mar 18.
It remains a significant challenge for fast and high-throughput detection of trace analytes in complex samples with surface-enhanced Raman scattering (SERS) strategy due to the severe interference from matrices. In this work, a miniaturized array gas membrane separation (AGMS) device coupled with SERS was designed and drew up to eliminate matrix influence and improve the reproducibility of SERS signal during real sample analysis. The design of miniaturized AGMS tube was optimized based on quantitative calculation of its air permeability by computational fluid dynamics simulation. A 10 mm height tube was selected as an optimized design with a recovery of 98.3% for acetaldehyde. The practical feasibility of miniaturized AGMS was validated based on the applications in biochemical analysis and food analysis, such as albuminuria and acetaldehyde in urine sample and metaldehyde and thiram in food samples. The results showed that SERS responses of all analytes dramatically increased by eliminating sample matrices after miniaturized AGMS process. Acetaldehyde, albuminuria, metaldehyde and thiram in real samples could be accurately quantified with recoveries of 82.0-123.3%, and the analytical results were validated by corresponding standard methods with relative error ranging from -4.8% to 5.3%. Time consumption of miniaturized AGMS-SERS for one real sample analysis including sample preparation and determination was less than 20 min and could treat 96 samples with 45 min in one run. It is potential that the miniaturized AGMS technique automated by implementation with a robotic arm could greatly expand the range and accelerate the speed of SERS analysis.
由于基质的严重干扰，基于表面增强拉曼散射（SERS）策略的复杂样品中痕量分析物的快速和高通量检测仍然是一个重大挑战。在这项工作中，设计并拟定了一种小型化阵列气膜分离（AGMS）装置与 SERS 结合，以消除基质影响并提高实际样品分析中 SERS 信号的重现性。基于计算流体动力学模拟对其透气性的定量计算，对小型化 AGMS 管的设计进行了优化。选择 10mm 高的管作为优化设计，乙醛的回收率为 98.3%。基于在生化分析和食品分析中的应用，如尿液样本中的白蛋白尿和乙醛以及食品样本中的多聚甲醛和福美双，验证了小型化 AGMS 的实际可行性。结果表明，通过小型化 AGMS 处理消除样品基质后，所有分析物的 SERS 响应都显著增强。实际样品中的乙醛、白蛋白尿、多聚甲醛和福美双可以通过准确定量，回收率为 82.0-123.3%，并通过相应的标准方法验证，相对误差在-4.8%至 5.3%之间。包括样品制备和测定在内，一个实际样品的小型化 AGMS-SERS 分析的总时间消耗小于 20 分钟，在一个运行中可以处理 96 个样品，时间为 45 分钟。通过实施机械臂自动化的小型化 AGMS 技术，有可能极大地扩大 SERS 分析的范围并加速其速度。
