School of chemistry, Sun Yat-sen University, Guangzhou 510006, China.
School of chemistry, Sun Yat-sen University, Guangzhou 510006, China.
J Chromatogr A. 2022 Jul 19;1675:463181. doi: 10.1016/j.chroma.2022.463181. Epub 2022 May 29.
Surface-enhanced Raman spectroscopy (SERS) is a powerful and promising analytical technique for fast, non-destructive, and sensitive analysis of trace analytes. However, the serious matrix interference effect of complex sample is a bottleneck that limited rapid SERS analysis. Therefore, it is essential to introduce proper sample preparation techniques to address the limitation for advancing SERS analysis. With adaptable sample preparation techniques, target substances could be separated and purified from complex matrices, matrix interference effect could be eliminated/reduced, and finally pure SERS spectra could be obtained. However, the process of traditional sample preparation usually requires the tedious and time-consuming operation, large solvent consumption, high labor intensity, as well as low efficiency. Given that SERS is an ultrafast analytical technique, there is a great desire to develop advanced/modern sample preparation techniques for fast and efficient analysis of complex samples. To attract much attention and raise research enthusiasm on the beginning, recent development on advanced sample preparation techniques for rapid SERS analysis of complex sample was reviewed. (1) 'All-in-one' strategy for simultaneous separation, enrichment, and in-situ SERS detection. (2) Integrated strategy for high-speed pretreatment and high throughput analysis. (3) Derivatization strategy switches on the SERS activity of molecules with weak responses. (4) Field-assisted strategy for preconcentration acceleration. (5) Instrument combination strategy for online processing and real-time SERS analysis. Finally, conclusions and perspectives on future development were briefly discussed.
表面增强拉曼光谱（SERS）是一种强大且有前途的分析技术，可用于快速、无损和灵敏地分析痕量分析物。然而，复杂样品的严重基质干扰效应是限制快速 SERS 分析的瓶颈。因此，引入适当的样品制备技术对于克服限制以推进 SERS 分析至关重要。通过适应性强的样品制备技术，可以将目标物质从复杂基质中分离和纯化，消除/减少基质干扰效应，最终获得纯净的 SERS 光谱。然而，传统的样品制备过程通常需要繁琐和耗时的操作、大量溶剂的消耗、高劳动强度以及低效率。鉴于 SERS 是一种超快的分析技术，人们非常希望开发先进/现代的样品制备技术，以实现对复杂样品的快速高效分析。为了引起人们对这一领域的关注并激发研究热情，本文综述了用于快速 SERS 分析复杂样品的先进样品制备技术的最新进展。（1）同时进行分离、富集和原位 SERS 检测的“一体化”策略。（2）用于高速预处理和高通量分析的集成策略。（3）衍生化策略可开启弱响应分子的 SERS 活性。（4）场辅助策略用于加速预浓缩。（5）用于在线处理和实时 SERS 分析的仪器组合策略。最后，简要讨论了结论和未来发展的展望。
