Department of Physical Therapy and Rehabilitation, College of Health Science, Eulji University, Seongnam, Republic of Korea.
Department of Physical Therapy and Rehabilitation, College of Health Science, Eulji University, Seongnam, Republic of Korea.
Biochem Biophys Res Commun. 2019 Jun 11;513(4):990-996. doi: 10.1016/j.bbrc.2019.04.027. Epub 2019 Apr 17.
The repair of articular cartilage needs a sufficient number of chondrocytes to replace the defect tissue. Direct reprogramming of fibroblasts into chondrocytes can provide a sufficient number of chondrocytes because fibroblasts can be expanded efficiently. Herein, we demonstrate for the first time that electrical stimulation can drive direct reprogramming of human dermal fibroblasts (HDFs) into hyaline chondrogenic cells. Our results shows that electrical stimulation drives condensation of HDFs and then enhances expression levels of chondrogenic markers, such as type II collagen, aggrecan, and Sox9, and decreases type I collagen levels without the addition of exogenous growth factors or gene transduction. Electrical stimulation-directly reprogrammed chondrogenic cells showed the normal karyotype. It was also found that electrical stimulation increased the secretion levels of TGF-beta1, PDGF-AA, and IGFBP-2, 3. These findings may contribute to not only novel approach of direct reprogramming but also cell therapy for cartilage regeneration.
关节软骨的修复需要足够数量的软骨细胞来替代缺损组织。成纤维细胞直接重编程为软骨细胞可以提供足够数量的软骨细胞，因为成纤维细胞可以有效地扩增。在此，我们首次证明电刺激可以驱动人真皮成纤维细胞（HDF）直接重编程为透明软骨细胞。我们的结果表明，电刺激驱动 HDF 凝聚，然后增强软骨形成标志物（如 II 型胶原、聚集蛋白聚糖和 Sox9）的表达水平，并降低 I 型胶原水平，而无需添加外源性生长因子或基因转导。电刺激直接重编程的软骨细胞显示出正常的核型。还发现电刺激增加了 TGF-β1、PDGF-AA 和 IGFBP-2 的分泌水平。这些发现不仅可能有助于直接重编程的新方法，也可能有助于软骨再生的细胞治疗。
