Jacob G S, Garbow J R, Schaefer J, Kishore G M
J Biol Chem. 1987 Feb 5;262(4):1552-7.
Lyophilized samples of Pseudomonas sp. PG2982 grown on 13C- and 15N-labeled glyphosate have been analyzed by single and double cross-polarization 13C NMR. Both the carbon and nitrogen metabolism of glyphosate are significantly influenced by the nitrogen source used for the growth of the organism. When ammonium sulfate is the source of nitrogen, the glycyl moiety of glyphosate is utilized intact for the biosynthesis of purines and proteins. But when the organism is grown on glycine as the source of nitrogen, the carbons and nitrogen of glyphosate are scrambled, consistent with incorporation into serine and pyruvate, and hence participation in general metabolism. When both ammonium and glycine are present in the growth medium, regulation of the metabolic fluxes along each of the two major pathways appears to be determined by the intracellular glycine concentration.
对在13C和15N标记的草甘膦上生长的嗜麦芽窄食单胞菌PG2982冻干样品进行了单重和双重交叉极化13C NMR分析。草甘膦的碳代谢和氮代谢均受到用于该生物体生长的氮源的显著影响。当硫酸铵作为氮源时，草甘膦的甘氨酰部分完整地用于嘌呤和蛋白质的生物合成。但当该生物体以甘氨酸作为氮源生长时，草甘膦的碳和氮会发生重排，这与掺入丝氨酸和丙酮酸并因此参与一般代谢一致。当生长培养基中同时存在铵和甘氨酸时，两条主要途径中每条途径的代谢通量调节似乎由细胞内甘氨酸浓度决定。
