Fitzgibbon J, Braymer H D
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
Appl Environ Microbiol. 1988 Jul;54(7):1886-8. doi: 10.1128/aem.54.7.1886-1888.1988.
Pseudomonas sp. strain PG2982 has the ability to use the phosphonate herbicide, glyphosate, as a sole phosphorus source (J. K. Moore, H. D. Braymer, and A. D. Larson, Appl. Environ. Microbiol. 46:316-320, 1983). Glyphosate uptake is maximal in the late log phase of growth and is induced by phosphate starvation. Uptake is inhibited by phosphate and arsenate, but not by the amino acids glycine and sarcosine. The Km and Vmax for glyphosate uptake were calculated to be 23 microM and 0.97 nmol/mg (dry weight) per min, respectively. A phosphate transport system with a broad substrate specificity may be responsible for glyphosate uptake.
假单胞菌属菌株PG2982能够将膦酸酯类除草剂草甘膦用作唯一的磷源（J.K.摩尔、H.D.布雷默和A.D.拉尔森，《应用与环境微生物学》46:316 - 320，1983年）。草甘膦的摄取在生长对数后期达到最大值，且由磷酸盐饥饿诱导。摄取受到磷酸盐和砷酸盐的抑制，但不受氨基酸甘氨酸和肌氨酸的抑制。草甘膦摄取的米氏常数（Km）和最大反应速率（Vmax）经计算分别为23微摩尔和每分钟0.97纳摩尔/毫克（干重）。一种具有广泛底物特异性的磷酸盐转运系统可能负责草甘膦的摄取。
