Metabolic Disorders Research Center, Department of Biochemistry and Biophysics, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Clin Chim Acta. 2017 Nov;474:34-37. doi: 10.1016/j.cca.2017.08.037. Epub 2017 Sep 1.
Type 2 diabetes mellitus (T2DM) is one of the diseases which depend on the obesity associated with insulin resistance. Various factors, such as a reduction in the activity of paraoxonase-1 enzyme (PON1), could affect T2DM. PON1 is a multifunctional enzyme with paraoxonase and arylesterase activities. The Activity of PON1 is influenced by various SNPs. The aim of presence study is to investigate the association between promoter polymorphism (-108C>T) of PON1 gene and its paraoxonase activity in patients suffering from Type 2 diabetes in Golestan Province, north of Iran. To this end, genomic DNA was extracted from whole blood then genotyping was carried out using PCR-RFLP and enzymatic activity of paraoxonase was measured in the serum of 90 healthy subjects and 90 diabetic patients. Data was analyzed using SPSS version 16. The relationship between the level of paraoxonase activity with polymorphisms of CC, CT, and TT was statistically significant in patients with T2DM. There was a significant association between polymorphism C-108>T of PON1 and type2 diabetes mellitus, with 24.4% of control group subjects and 15.5% of patients having CC genotype; p<0.05. The ratio for CT genotype in target and control groups was 51.1% and 61.1% respectively; p<0.05. TT genotype was 33.3% in patients and 14.4% in the control group; p<0.05. In the present study, the highest frequency belonged to CT genotype in both the control and the target groups, followed by CC genotype in control group and TT genotype in target group. Our findings revealed that paraoxonase activity of PON1 in the control group was significantly higher in comparison with diabetic patients.
2 型糖尿病（T2DM）是一种与肥胖相关的胰岛素抵抗疾病。各种因素，如对氧磷酶-1 酶（PON1）活性的降低，可能会影响 T2DM。PON1 是一种具有对氧磷酶和芳基酯酶活性的多功能酶。PON1 的活性受多种 SNPs 的影响。本研究的目的是探讨伊朗北部戈勒斯坦省 2 型糖尿病患者 PON1 基因启动子多态性（-108C>T）与其对氧磷酶活性的关系。为此，从全血中提取基因组 DNA，然后使用 PCR-RFLP 进行基因分型，并在 90 名健康受试者和 90 名糖尿病患者的血清中测量对氧磷酶的活性。数据采用 SPSS 版本 16 进行分析。PON1 活性水平与 CC、CT 和 TT 多态性的关系在 T2DM 患者中具有统计学意义。PON1 的 C-108>T 多态性与 2 型糖尿病之间存在显著关联，对照组受试者中有 24.4%和 15.5%的患者为 CC 基因型；p<0.05。目标组和对照组 CT 基因型的比例分别为 51.1%和 61.1%；p<0.05。TT 基因型在患者中为 33.3%，在对照组中为 14.4%；p<0.05。在本研究中，对照组和目标组中 CT 基因型的频率最高，其次是对照组中的 CC 基因型和目标组中的 TT 基因型。我们的研究结果表明，PON1 的对氧磷酶活性在对照组中明显高于糖尿病患者。
