Applied Physiology & Nutrition Research Group, Laboratory of Assessment and Conditioning in Rheumatology, School of Physical Education and Sport, School of Medicine, University of São Paulo, Brazil.
Clinical Oncology Service, Instituto do Câncer do Estado de São Paulo, Brazil.
Cytokine. 2019 Aug;120:66-70. doi: 10.1016/j.cyto.2019.04.006. Epub 2019 Apr 18.
Polycystic ovary syndrome (PCOS) is characterized by exacerbated inflammation, which is implicated in cardiometabolic dysfunction. This study aimed to examine the potential effects of acute exercise on inflammatory responses in obese/overweight PCOS women and their controls. Participants underwent a single bout of moderate-intensity aerobic exercise (30 min at ∼65% of VO). Blood and muscle samples were collected immediately before (PRE) and 60 min after the exercise session. Cytokines (i.e., IL-1β, IL-6, IL-4, IL-10, TNF-α) were measured both in plasma and in skeletal muscle, and proteins related to inflammatory signaling (IKKα/β and JNK) were assessed in skeletal muscle. At PRE, PCOS showed elevated muscle TNF-α (+62%, p = 0.0012) and plasma IL-1β (+76%, p = 0.0010) compared to controls. In PCOS, exercise decreased plasma and muscle TNF-α (-14%, p = 0.0003 and -46%, p = 0.0003), as well as increased plasma and muscle IL-4 (+147%, p = 0.0018 and +62%, p = 0.0474) and plasma IL-10 (+38%, p = 0.0029). Additionally, IKKα/β and JNK phosphorylation in skeletal muscle, which was higher in PCOS at PRE, was significantly reduced by exercise (-58%, p < 0.0001 and -46%, p < 0.0001, respectively), approaching control levels. Person's correlations between PRE values and delta changes (i.e., exercise effect) showed significant, negative associations for plasma IL-1β (r = -0.92, p < 0.0001), TNF-α (r = -0.72, p = 0.0100) and IL-6 (r = -0.58, p = 0.05), and muscle TNF-α (r = -0.95, p < 0.0001), IKKα/β (r = -0.75, p = 0.005), and JNK (r = -0.94, p < 0.0001) in PCOS. In conclusion, exercise can mitigate the inflammatory milieu in women with PCOS. The anti-inflammatory role of exercise could underlie its cardiometabolic protection in PCOS.
多囊卵巢综合征（PCOS）的特征是炎症加剧，这与心脏代谢功能障碍有关。本研究旨在探讨急性运动对肥胖/超重 PCOS 女性及其对照组炎症反应的潜在影响。参与者进行了一次中等强度的有氧运动（30 分钟，约 65%的 VO）。在运动前（PRE）和运动后 60 分钟，采集血液和肌肉样本。在血浆和骨骼肌中均测量了细胞因子（即 IL-1β、IL-6、IL-4、IL-10、TNF-α），并评估了骨骼肌中与炎症信号相关的蛋白（IKKα/β 和 JNK）。在 PRE，与对照组相比，PCOS 显示出肌肉 TNF-α（增加 62%，p=0.0012）和血浆 IL-1β（增加 76%，p=0.0010）升高。在 PCOS 中，运动降低了血浆和肌肉 TNF-α（分别减少 14%，p=0.0003 和减少 46%，p=0.0003），并增加了血浆和肌肉 IL-4（分别增加 147%，p=0.0018 和增加 62%，p=0.0474）和血浆 IL-10（增加 38%，p=0.0029）。此外，在 PRE，PCOS 中骨骼肌 IKKα/β 和 JNK 磷酸化更高，运动后显著降低（分别减少 58%，p<0.0001 和减少 46%，p<0.0001），接近对照组水平。参与者 PRE 值与 delta 变化（即运动效应）之间的 Person 相关性显示，血浆 IL-1β（r=-0.92，p<0.0001）、TNF-α（r=-0.72，p=0.0100）和 IL-6（r=-0.58，p=0.05）以及肌肉 TNF-α（r=-0.95，p<0.0001）、IKKα/β（r=-0.75，p=0.005）和 JNK（r=-0.94，p<0.0001）与 PCOS 呈显著负相关。总之，运动可以减轻 PCOS 女性的炎症环境。运动的抗炎作用可能是其在 PCOS 中心血管代谢保护作用的基础。
