Budd R C, Cerottini J C, MacDonald H R
J Immunol. 1987 Feb 15;138(4):1009-13.
Murine peripheral Lyt-2+ T cells could be subdivided according to surface expression of the Pgp-1 glycoprotein into major (71%) Pgp-1- and minor (29%) Pgp-1+ subsets. A striking correlation was observed between Pgp-1 expression and enrichment for antigen-specific memory cytolytic T lymphocyte precursors (CTLp). After immunization with the male minor transplantation antigen H-Y, virtually all the H-Y-specific CTLp were found in the minor Pgp-1+ subset of Lyt-2+ cells. In addition, after alloimmunization the frequency of allospecific CTLp resistant to inhibition by anti-Lyt-2 antibody was markedly enriched within the Pgp-1+ cells, suggesting an enrichment for CTLp bearing high avidity antigen receptors. Taken together, these data suggest that surface Pgp-1 expression is stably acquired at the time of primary antigenic stimulation by virgin T cells. As such, Pgp-1 represents an important marker for identifying a subset of Lyt-2+ T cells with the quantitative and qualitative properties of memory CTLp.
小鼠外周Lyt-2⁺ T细胞可根据Pgp-1糖蛋白的表面表达分为主要的（71%）Pgp-1⁻亚群和次要的（29%）Pgp-1⁺亚群。观察到Pgp-1表达与抗原特异性记忆性细胞毒性T淋巴细胞前体（CTLp）的富集之间存在显著相关性。在用雄性次要移植抗原H-Y免疫后，几乎所有H-Y特异性CTLp都存在于Lyt-2⁺细胞的次要Pgp-1⁺亚群中。此外，同种异体免疫后，对抗Lyt-2抗体抑制有抗性的同种特异性CTLp频率在Pgp-1⁺细胞中显著富集，表明具有高亲和力抗原受体的CTLp得到了富集。综上所述，这些数据表明，表面Pgp-1表达是初始T细胞在初次抗原刺激时稳定获得的。因此，Pgp-1是鉴定具有记忆CTLp定量和定性特性的Lyt-2⁺ T细胞亚群的重要标志物。
