Department of Pharmacognosy and Biotechnology,Faculty of Pharmacy, Mazandaran University of Medical Sciences,Sari,Iran.
Department of Parasitology and Mycology,School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Parasitology. 2019 Aug;146(9):1188-1198. doi: 10.1017/S0031182019000325. Epub 2019 Apr 22.
Giardia lamblia (G. lamblia) is the most widely known protozoan parasite that causes human gastrointestinal infection worldwide. Some natural compounds exhibited pivotal effects against different infectious diseases. In this research, the antigiardial activity and cytotoxicity of fungal chitosan, nano-chitosan, Rhamnus cathartica (R. cathartica) and emodin were evaluated in Balb/c mice. Genotyping of G. lamblia was assessed by PCR-RFLP technique. Different concentrations of mentioned compounds were used to check their antigiardial and cytotoxicity effects on human intestinal epithelial cells (HT-29) after 24, 48 and 72 h. The G. lamblia strain used in the current work was genotyped and revealed as an AII assemblage. All the concentration showed acceptable activity against G. lamblia cysts and trophozoites in comparison to the negative and positive controls (furazolidone and metronidazole) in vitro (P 0.05). The maximum mortality rate (100%) was achieved at 100 and 50 µg kg-1 concentrations after 48 and 72 h of exposure time, respectively. Our results provide significant information about the new antigiardial agent and proposed the nano-chitosan and emodin for the development of new drugs against G. lamblia in the future.
蓝氏贾第鞭毛虫（G. lamblia）是全球范围内导致人类胃肠道感染的最广为人知的原生动物寄生虫。一些天然化合物对不同的传染病表现出关键作用。在这项研究中，在 Balb/c 小鼠中评估了真菌壳聚糖、纳米壳聚糖、鼠李（R. cathartica）和大黄素的抗贾第鞭毛虫活性和细胞毒性。通过 PCR-RFLP 技术评估了蓝氏贾第鞭毛虫的基因分型。在 24、48 和 72 小时后，用不同浓度的上述化合物检查它们对人肠上皮细胞（HT-29）的抗贾第鞭毛虫和细胞毒性作用。目前工作中使用的蓝氏贾第鞭毛虫菌株经过基因分型，被鉴定为 AII 组合。与阴性和阳性对照（呋喃唑酮和甲硝唑）相比，所有浓度在体外均显示出对贾第鞭毛虫包囊和滋养体的可接受活性（P 0.05）。在暴露 48 和 72 小时后，分别在 100 和 50 μg kg-1 浓度下达到最大死亡率（100%）。我们的结果提供了有关新型抗贾第鞭毛虫剂的重要信息，并提出纳米壳聚糖和大黄素用于未来开发针对 G. lamblia 的新药。
