体外研究评估迷迭香乙醇提取物及其壳聚糖纳米粒子对鸡柔嫩艾美耳球虫卵囊的功效。
In Vitro Study to Evaluate the Efficacy of Ultrasonicated Ethanolic Extract of Rosmarinus officinalis and its Chitosan-Based Nanoparticles Against Eimeria tenella Oocysts of Chickens.
机构信息
Zoology Department, Faculty of Science, Kafrelsheikh University, Kafr ElSheikh, 33516, Egypt.
Zoology Department, Faculty of Science, Tanta University, EL Gharbia, 31527, Egypt.
出版信息
AAPS PharmSciTech. 2022 Nov 3;23(8):295. doi: 10.1208/s12249-022-02445-z.
In this study, chitosan nanoparticles (CsNPs) were used as nanocarrier for ultrasonicated ethanolic extract of Rosmarinus officinalis (UEERO) as a new nanoformulation against Eimeria tenella. Herein, CsNPs have been synthesized by ionic gelation method at pH 3 (CsNPs3) and pH 5 (CsNPs5), followed by characterization of morphology, size, polydispersity index (PDI), surface charge, and loading efficiency of UEERO. An in vitro sporulation inhibition assay (10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 0.07, 0.04, 0.02, and 0.01 mg/ml normal saline solution) against E. tenella was conducted. Results showed that free CsNPs and UEERO-CsNPs3/5 were cubic- and spherical-shaped with positive charge and average size of ~ 150.8 nm (314.4 nm) and 151.7 nm (321.1 nm), respectively. The total loading efficiency using UV-vis spectrophotometer, was 80.05 at pH 5 and 64.39% at pH 3. The in vitro sporulation inhibition assay revealed that UEERO, CsNPs3/5, and UEERO-CsNPs3/5 showed a potential inhibitory effect on sporulation (%), distortion in wall (%), and sporocyst abnormality (%) in a dose-dependent manner. Accordingly, the concentration (10 mg/ml) showed the best efficacy after 24 h in UEERO, free CsNPs, and UEERO-CsNPs. Moreover, UEERO-CsNPs3 and UEERO-CsNPs5 had stopped the sporulation (%) after 72 h. Taken all together, UEERO-CsNPs3 and UEERO-CsNPs5 are best effective against E. tenella in a dose-dependent manner in terms of sporulation (%), distortion in wall (%), and sporocysts abnormality.
在这项研究中，壳聚糖纳米粒子（CsNPs）被用作迷迭香乙醇提取物（UEERO）的超声纳米载体，作为一种针对柔嫩艾美耳球虫的新型纳米制剂。在此，通过离子凝胶法在 pH3（CsNPs3）和 pH5（CsNPs5）下合成了 CsNPs，随后对其形态、粒径、多分散指数（PDI）、表面电荷和 UEERO 的载药量进行了表征。进行了体外孢子抑制试验（10、5、2.5、1.25、0.62、0.31、0.15、0.07、0.04、0.02 和 0.01 mg/ml 生理盐水），以对抗柔嫩艾美耳球虫。结果表明，游离 CsNPs 和 UEERO-CsNPs3/5 分别呈立方体形和球形，带正电荷，平均粒径分别为~150.8nm（314.4nm）和 151.7nm（321.1nm）。使用紫外可见分光光度计测定的总载药量分别为 pH5 时为 80.05%，pH3 时为 64.39%。体外孢子抑制试验表明，UEERO、CsNPs3/5 和 UEERO-CsNPs3/5 对孢子形成（%）、壁变形（%）和孢子畸形（%）具有潜在的抑制作用，呈剂量依赖性。因此，在 24 小时后，UEERO、游离 CsNPs 和 UEERO-CsNPs 浓度（10mg/ml）显示出最佳疗效。此外，UEERO-CsNPs3 和 UEERO-CsNPs5 在 72 小时后停止了孢子形成（%）。综上所述，UEERO-CsNPs3 和 UEERO-CsNPs5 在抑制柔嫩艾美耳球虫孢子形成（%）、壁变形（%）和孢子畸形方面具有最佳疗效，呈剂量依赖性。
Zotero 插件