Department of Oral Science, Kanagawa Dental University Graduate School of Dentistry, Yokosuka, Japan; Department of Oral Interdisciplinary Medicine, Kanagawa Dental University Graduate School of Dentistry, Yokosuka, Japan.
Department of Oral Science, Kanagawa Dental University Graduate School of Dentistry, Yokosuka, Japan.
Ultrasound Med Biol. 2019 Jul;45(7):1721-1732. doi: 10.1016/j.ultrasmedbio.2019.02.015. Epub 2019 Apr 18.
We developed a rat model of bisphosphonate-related osteonecrosis of the jaw (BRONJ) by removing a maxillary molar tooth (M1) from ovariectomized rats after treatment with alendronate. To mimic periodontitis, some of the rats were administered Porphyromonas gingivalis (p. gingivalis) at the M1 site every 2 to 3 d for 2 wk. Rats pretreated with alendronate plus p. gingivalis showed delayed healing of socket epithelia, periosteal reaction of alveolar bone formation and lower bone mineral density in the alveolus above adjacent M2 teeth. These abnormalities were prevented by tooth socket exposure to 20 min/d low-intensity pulsed ultrasound (LIPUS), which restored diminished expression of RANKL, Bcl-2, IL-6, Hsp70, NF-κB and TNF-α messenger ribonucleic acids in remote bone marrow, suggesting LIPUS prevented development of BRONJ-like pathophysiology in rat by inducing systemic responses for regeneration, in addition to accelerating local healing. Non-invasive treatment by LIPUS, as well as low-level laser therapy, may be useful for medication-related osteonecrosis of the jaw patients.
我们通过在给予阿伦膦酸盐治疗后从去卵巢大鼠中去除上颌磨牙（M1）来开发骨坏死相关的双膦酸盐性颌骨坏死（BRONJ）的大鼠模型。为了模拟牙周炎，一些大鼠每隔 2-3 天在 M1 部位给予牙龈卟啉单胞菌（p. gingivalis）2 周。用阿伦膦酸盐加 p. gingivalis预处理的大鼠表现出牙槽窝上皮愈合延迟、骨膜反应性牙槽骨形成和相邻 M2 牙齿上方牙槽骨骨密度降低。这些异常通过将牙窝暴露于 20 分钟/天的低强度脉冲超声（LIPUS）来预防，LIPUS 恢复了远程骨髓中 RANKL、Bcl-2、IL-6、Hsp70、NF-κB 和 TNF-α信使核糖核酸表达的减少，表明 LIPUS 通过诱导系统反应促进再生，除了加速局部愈合外，还可预防大鼠类似 BRONJ 的病理生理学的发展。LIPUS 的非侵入性治疗以及低水平激光治疗可能对药物相关的颌骨坏死患者有用。
