Department of Conservative Dentistry and Oral Science Research Center, Yonsei University College of Dentistry, Seoul, 03722, Republic of Korea.
College of Dentistry, Dankook University, Cheonan, 31116, Republic of Korea.
Sci Rep. 2021 Jun 18;11(1):12892. doi: 10.1038/s41598-021-92471-x.
This study aimed to investigate the effects of low-intensity pulsed ultrasound (LIPUS) on replacement root resorption after replantation of avulsed teeth stored in a dry condition in dogs. A total of 73 premolar roots from four male mongrel dogs were intentionally avulsed with forceps and divided into four groups-HN, HL, DN, and DL-according to storage conditions and whether or not they received LIPUS treatment. Thirty-eight roots were kept in Hanks' Balanced Salt Solution for 30 min (HN and HL groups), whereas the remaining 35 roots were left to dry in the air for an hour (DN and DL groups) prior to replantation. Following replantation, the roots in the HL and DL groups (21 and 18 roots, respectively) received a 20-min daily LIPUS treatment for 2 weeks. The animals were euthanized 4 weeks after the operation. Micro-computed tomography images were acquired for each root and the amount of replacement root resorption was measured three-dimensionally. Histological assessments were also carried out. There was significantly less replacement root resorption for the roots in the DL group compared to the DN group (p < 0.01). Histological findings in the DN group demonstrated evident replacement root resorption, whereas the DL group revealed less severe resorption compared to the DN group. Within the limitations, these results suggest that LIPUS could attenuate the replacement resorption of avulsed teeth stored in a dry condition, thereby improving their prognosis.
本研究旨在探讨低强度脉冲超声（LIPUS）对干燥保存离体牙再植后替代性根吸收的影响。共 73 颗来自 4 只雄性杂种犬的前磨牙根，用镊子将其故意拔出，根据储存条件和是否接受 LIPUS 治疗，将其分为四组-HN、HL、DN 和 DL。38 颗根保存在 Hank's 平衡盐溶液中 30 分钟（HN 和 HL 组），而其余 35 颗根在空气中干燥 1 小时（DN 和 DL 组）后再植。再植后，HL 和 DL 组（分别为 21 根和 18 根）的根每天接受 20 分钟的 LIPUS 治疗，持续 2 周。术后 4 周处死动物。对每个根进行微计算机断层扫描图像采集，并三维测量替代性根吸收的量。还进行了组织学评估。与 DN 组相比，DL 组的替代性根吸收明显减少（p < 0.01）。DN 组的组织学发现显示明显的替代性根吸收，而 DL 组与 DN 组相比，吸收程度较轻。在一定限制内，这些结果表明，LIPUS 可以减轻干燥保存的离体牙的替代性根吸收，从而改善其预后。
