Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, R. Dr. Silvio Henrique Braune, 22 Centro, Nova Friburgo, Rio de Janeiro, 22625-650, Brazil.
Clin Oral Investig. 2021 Dec;25(12):6653-6659. doi: 10.1007/s00784-021-03951-3. Epub 2021 Apr 25.
The aim of this study was to evaluate the preventive effect of ozonated oil on the development of MRONJ-like lesions at tooth extraction sites in rats submitted to zoledronic acid treatment.
Twelve rats were divided into two groups. Each rat received an injection of zoledronic acid solution once a week for 5 weeks before having its upper first molar extracted. In group 1, the clotted socket was maintained, while in the group 2, the socket was treated with ozonated oil for 10 min/day during 3 days. After euthanasia, block resection was performed for histological analysis. Necrotic bone was defined as the area of the bone with empty osteocyte lacunae and vital bone as the area with osteocyte-occupied lacunae.
There was no statistically significant difference between the groups in the average area of vital bone (p = 0.2248) and the average area of necrotic bone (p = 0.1208). However, the average area of vital bone in group 1 was smaller (24.1 ± 2.9 cells/cm) than in group 2 (26.8 ± 4.2 cells/cm), and the average area of necrotic bone in group 1 was higher (7.0 ± 2.5 cells/cm) than in group 2 (4.0 ± 1.1 cells/cm), indicating that ozonated oil may reduce the development of osteonecrosis.
Our findings suggest that ozonated oil might prevent MRONJ-like lesions at tooth extraction sites in rats submitted to a disease induction protocol. Further research should be conducted to confirm this hypothesis.
MRONJ has been reported as a complication of surgical dental procedures, and there are currently no predictable preventive therapies.
本研究旨在评估臭氧油对接受唑来膦酸治疗的大鼠拔牙部位 MRONJ 样病变发展的预防作用。
12 只大鼠被分为两组。每组大鼠每周接受唑来膦酸溶液注射一次，共 5 周，然后拔除上颌第一磨牙。在第 1 组中，保持血凝块牙槽窝，而在第 2 组中，每天用臭氧油处理 10 分钟，持续 3 天。安乐死后进行块状切除进行组织学分析。坏死骨定义为骨内有空的骨细胞陷窝的区域，活骨定义为有骨细胞占据陷窝的区域。
两组之间活骨的平均面积（p=0.2248）和坏死骨的平均面积（p=0.1208）无统计学差异。然而，第 1 组的活骨平均面积（24.1±2.9 个细胞/cm）小于第 2 组（26.8±4.2 个细胞/cm），第 1 组的坏死骨平均面积（7.0±2.5 个细胞/cm）大于第 2 组（4.0±1.1 个细胞/cm），表明臭氧油可能减少骨坏死的发生。
我们的研究结果表明，臭氧油可能预防接受疾病诱导方案的大鼠拔牙部位发生 MRONJ 样病变。应进一步开展研究以证实这一假设。
MRONJ 已被报道为牙科手术的并发症，目前尚无可预测的预防疗法。
