a Department of Stem Cell Biology, Institute for Research and Medical Consultations , Imam Abdulrahman Bin Faisal University , Dammam , Saudi Arabia.
b Department of Biophysics, Institute for Research and Medical Consultations , Imam Abdulrahman Bin Faisal University , Dammam , Saudi Arabia.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1533-1542. doi: 10.1080/21691401.2019.1577886.
Poly (methyl methacrylate) (PMMA) is basically biocompatible polyester with high resistance to chemical hydrolysis, and high drug permeability and the most important characteristics of PMMA is that it does not produce any toxicity. There is not much information about PMMA action on the colon cancer cells. In the present study, we have synthesized PMMA nanoparticles. The distribution pattern of PMMA particles was analysed by Zeta sizer and the size of the particles was calculated by using quasi elastic light scattering (QELS). The surface structure and the morphology of PMMA were characterized by transmission electron microscope (TEM) and scanning electron microscope (SEM), respectively. We have also analysed their effects on cancerous cells (human colorectal carcinoma cells, HCT-116) and normal, healthy cells (human embryonic kidney cells, HEK-293) by using morphometric, MTT, DAPI and wound healing methods. We report that PMMA particles inhibited the cancer cell viability in a dose-dependent manner. The lower dose (1.0 μg/ml) showed a moderate decrease in cancer cell viability, whereas higher dosages (2.5 μg/ml, 5.0 μg/mL and 7.5 μg/mL) showed steadily decrease in the cancer cell viability. We also report that PMMA is highly selective to cancerous cells (HCT-116), as we did not find any action on the normal healthy cells (HEK-293). In conclusion, our results suggest PMMA particles are potential biomaterials to be used in the treatment of colon cancer.
聚甲基丙烯酸甲酯（PMMA）基本上是一种具有高耐化学水解性、高药物渗透性的生物相容性聚酯，而 PMMA 最重要的特性是它不会产生任何毒性。关于 PMMA 对结肠癌细胞的作用，目前的信息还不多。在本研究中，我们合成了 PMMA 纳米颗粒。通过 Zeta 粒径仪分析 PMMA 颗粒的分布模式，并通过准弹性光散射（QELS）计算颗粒的大小。通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）分别对 PMMA 的表面结构和形态进行了表征。我们还通过形态计量学、MTT、DAPI 和划痕愈合方法分析了它们对癌细胞（人结肠癌细胞，HCT-116）和正常健康细胞（人胚肾细胞，HEK-293）的影响。我们报告称，PMMA 颗粒以剂量依赖性方式抑制癌细胞活力。较低剂量（1.0μg/ml）表现出中等程度的癌细胞活力降低，而较高剂量（2.5μg/ml、5.0μg/ml 和 7.5μg/ml）则表现出癌细胞活力的稳步降低。我们还报告称，PMMA 对癌细胞（HCT-116）具有高度选择性，因为我们没有发现它对正常健康细胞（HEK-293）有任何作用。总之，我们的结果表明 PMMA 颗粒是一种有潜力的生物材料，可用于结肠癌的治疗。
