超声合成与 MnZnErYFeO 纳米粒子的生物医学应用。

Ultrasonic Synthesis and Biomedical Application of MnZnErYFeO Nanoparticles.

机构信息

Department of Epidemic Diseases Research, Institute for Research & Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, 31441 Dammam, Saudi Arabia.

Department of Biophysics, Institute for Research and Medical Consultations, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, 31441 Dammam, Saudi Arabia.

出版信息

Biomolecules. 2021 May 8;11(5):703. doi: 10.3390/biom11050703.

DOI:10.3390/biom11050703

PMID:34066897

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8150661/

Abstract

In the present study, biocompatible manganese nanoparticles have been linked with zinc and iron molecules to prepare different derivatives of MnZnErYFeO NPs (x = 0.02, 0.04, 0.06, 0.08, 0.10), using an ultrasonication approach. The structure, surface morphology, and chemical compositions of MnZnErYFeO NPs were elucidated by X-ray diffractometer (XRD), High-resolution transmission electron microscopy (HR-TEM), scanning electron microscope (SEM), and Energy Dispersive X-Ray Analysis (EDX) techniques. The bioactivity of MnZnErYFeO NPs on normal (HEK-293) and (HCT-116) colon cancer cell line was evaluated. The MnZnErYFeO NPs treatment post 48 h resulted in a significant reduction in cells (via MTT assay, having an IC value between 0.88 µg/mL and 2.40 µg/mL). The specificity of MnZnErYFeO NPs were studied by treating them on normal cells line (HEK-293). The results showed that MnZnErYFeO NPs did not incur any effect on HEK-293, which suggests that MnZnErYFeO NPs selectively targeted the colon cancerous cells. Using , antifungal activity was also studied by evaluating minimum inhibitory/fungicidal concentration (MIC/MFC) and the effect of nanomaterial on the germ tube formation, which exhibited that NPs significantly inhibited the growth and germ tube formation. The obtained results hold the potential to design nanoparticles that lead to efficient bioactivity.

摘要

在本研究中，通过超声法将生物相容性的锰纳米粒子与锌和铁分子结合，制备了不同的 MnZnErYFeO NPs（x=0.02、0.04、0.06、0.08、0.10）衍生物。通过 X 射线衍射仪（XRD）、高分辨率透射电子显微镜（HR-TEM）、扫描电子显微镜（SEM）和能量色散 X 射线分析（EDX）技术阐明了 MnZnErYFeO NPs 的结构、表面形貌和化学成分。评估了 MnZnErYFeO NPs 对正常（HEK-293）和（HCT-116）结肠癌细胞系的生物活性。MnZnErYFeO NPs 处理 48 小时后，细胞显著减少（通过 MTT 测定，IC 值在 0.88 µg/mL 和 2.40 µg/mL 之间）。通过用 MnZnErYFeO NPs 处理正常细胞系（HEK-293）来研究 MnZnErYFeO NPs 的特异性。结果表明，MnZnErYFeO NPs 对 HEK-293 没有任何影响，这表明 MnZnErYFeO NPs 选择性地针对结肠癌细胞。通过评估最小抑菌/杀菌浓度（MIC/MFC）和纳米材料对芽管形成的影响，也研究了抗真菌活性，结果表明 NPs 显著抑制了生长和芽管形成。研究结果具有设计高效生物活性纳米材料的潜力。