Fang Zhiming, Li Chengren, Li Shouchao
Department of Anus and Intestine Surgery, Weifang People's Hospital, Weifang, Shandong 261000, P.R. China.
Exp Ther Med. 2019 May;17(5):3921-3928. doi: 10.3892/etm.2019.7435. Epub 2019 Mar 26.
To explore microRNA (miR)-193b expression and its potential role in colon cancer, reverse transcription-quantitative polymerase chain reaction was performed to detect the miR-193b expression levels in 62 colon cancer tissues and normal adjacent tissues. The miR-193b-overexpressed cell line SW620 was used to study the role of miR-193b in colon cancer. Subsequently, a Transwell assay and cell cycle assay were performed to observe the functional cell changes in the expression levels of miR-193b. Results indicated that miR-193b expression levels were significantly decreased in colon cancer tissues compared with adjacent normal tissue (P<0.001) and the expression of miR-193b was significantly correlated with TNM staging (P=0.03) and lymph node invasion (P=0.007). Furthermore, overexpression of miR-193b significantly decreased colon cancer cell cycle progression and its migration ability. In addition, the present findings suggested that the increased expression of miR-193b by RAB22A, inhibited downstream proteins involved in the Ras signaling pathway, including the Ras and extracellular signal-related kinase which may inhibit cancer proliferation and migration. In conclusion, the aim was to clarify the association of miR-193b expression with colon cancer, and to explore the mechanism of miR-193b in colon cancer proliferation and cell migration. The preliminary findings revealed that miR-193b may have an important role in the process in colon cancer cell cycle and migration by the RAB22A-Ras signaling pathway, thus providing a theoretical basis for miR-193b as a potential molecular target for colon cancer treatment.
为探究微小RNA（miR）-193b在结肠癌中的表达及其潜在作用，采用逆转录-定量聚合酶链反应检测62例结肠癌组织及癌旁正常组织中miR-193b的表达水平。使用miR-193b过表达的细胞系SW620研究miR-193b在结肠癌中的作用。随后，进行Transwell实验和细胞周期实验，以观察miR-193b表达水平变化时细胞的功能改变。结果表明，与癌旁正常组织相比，结肠癌组织中miR-193b表达水平显著降低（P<0.001），且miR-193b的表达与TNM分期（P=0.03）及淋巴结转移（P=0.007）显著相关。此外，miR-193b过表达显著降低了结肠癌细胞周期进程及其迁移能力。另外，本研究结果提示，RAB22A使miR-193b表达增加，抑制了Ras信号通路下游蛋白，包括Ras和细胞外信号调节激酶，这可能抑制了癌症的增殖和迁移。总之，本研究旨在阐明miR-193b表达与结肠癌的关联，并探究miR-193b在结肠癌增殖和细胞迁移中的作用机制。初步研究结果显示，miR-193b可能通过RAB22A-Ras信号通路在结肠癌细胞周期和迁移过程中发挥重要作用，从而为miR-193b作为结肠癌治疗的潜在分子靶点提供理论依据。
