Wang Ya-Nan, Chen Zhao-Hua, Chen Wei-Chang
The First Affiliated Hospital of Soochow University, Shizi Street #188, Suzhou, 215006, Jiangsu, People's Republic of China.
Suzhou Municipal Hospital, Suzhou Hospital Affiliated to Nanjing Medical University, Suzhou, 215002, Jiangsu, People's Republic of China.
Eur J Med Res. 2017 Nov 29;22(1):51. doi: 10.1186/s40001-017-0294-5.
To identify the expression profile of novel microRNAs (miRNAs) in colon cancer and evaluate their clinical applicability.
Differences in the expression of serum miRNAs in patients with colon cancer and healthy controls were identified using miRNA microarrays. Differentially expressed miRNAs were verified via real-time polymerase chain reaction (PCR) using sera from 50 patients with colon cancer and 44 healthy controls. These miRNAs were also verified in a double-blind validation experiment using sera from 30 patients with colon cancer, 30 patients with colonic polyps, and 30 healthy controls.
Microarray hybridization revealed that 87 miRNAs were differentially expressed between the sera of patients with colon cancer and healthy controls. Among these miRNAs, 39 miRNAs were up-regulated, whereas 48 miRNAs were down-regulated. Verification of the expression of these miRNAs using real-time PCR revealed that the expression levels of miR-31, miR-141, miR-224-3p, miR-576-5p, and miR-4669 were significantly different between patients with colon cancer and healthy controls. Using these five miRNAs to construct a miRNA expression profile (or miRNA panel) will facilitate more effective diagnosis of colon cancer.
Clinical analysis of miR-31, miR-141, miR-224-3p, miR-576-5p, and miR-4669 expression in patients with colon cancer may facilitate the diagnosis of colon cancer.
鉴定新型微小RNA（miRNA）在结肠癌中的表达谱，并评估其临床适用性。
使用miRNA微阵列鉴定结肠癌患者和健康对照者血清中miRNA表达的差异。使用来自50例结肠癌患者和44例健康对照者的血清，通过实时聚合酶链反应（PCR）验证差异表达的miRNA。还使用来自30例结肠癌患者、30例结肠息肉患者和30例健康对照者的血清，在双盲验证实验中对这些miRNA进行了验证。
微阵列杂交显示，结肠癌患者血清与健康对照者血清之间有87种miRNA差异表达。在这些miRNA中，39种miRNA上调，48种miRNA下调。使用实时PCR验证这些miRNA的表达，结果显示结肠癌患者与健康对照者之间miR-31、miR-141、miR-224-3p、miR-576-5p和miR-4669的表达水平存在显著差异。使用这五种miRNA构建miRNA表达谱（或miRNA组合）将有助于更有效地诊断结肠癌。
对结肠癌患者中miR-31、miR-141、miR-224-3p、miR-576-5p和miR-4669表达进行临床分析可能有助于结肠癌的诊断。
