Changizi Vahid, Haeri Seyed Abolghasem, Abbasi Sakineh, Rajabi Zahra, Mirdoraghi Mohammad
Department of Radiology and Radiotherapy Technology, School of Allied Health Sciences, Tehran University of Medical Sciences, Tehran, Iran.
Nuclear Science and Technology Research Institute, Tehran, Iran.
MethodsX. 2019 Apr 3;6:714-717. doi: 10.1016/j.mex.2019.03.020. eCollection 2019.
Radioprotectors by neutralizing the effects of free radicals, reduce the destructive effects of radiation. In this protocol article, the radioprotectory effect of vitamin A on micronuclei induced by gamma radiation was evaluated using micronucleus test. Vitamin A was injected intraperitoneally at 100 and 400 mg/kg two hours before 2 Gray (Gy) of gamma radiation. Animals were sacrificed after 24 h, and then specimens of the bone marrow were smeared and stained. The number of micronuclei were counted in polychromatic cells. Both dosage of vitamin A reduced the micronucleus in bone marrow polychromatic erythrocytes (MnPCE) level, which is statistically significant. The appropriate amount of vitamin A for protection in mice is 100 mg/kg, which protect the bone marrow of mice against clastogenic effects of radiation. The results of the study showed that vitamin A, possibly with an antioxidant mechanism, eliminates the effects of free radicals from ionizing radiation on bone marrow cells and reduces genetic damage. •The data of radioprotective effects of vitamin A showed that administration of 100 mg/kg vitamin A to mice prior to 2 Gy of gamma radiation has reduced the micronucleus levels in PCE cells by a factor of 2.62.•Administration of 100 mg/kg vitamin A, which is much smaller than LD50 of vitamin A (LD50 for intraperitoneal injection = 1510 ± 240 mg/kg) can protect mice.•Vitamin A reduces the harmful effects of ionizing radiation on DNA, due to the antioxidant activity and the trapping of free radicals produced by radiation, and diminish the genetic damage caused by radiation.•Vitamin A has no effect on the proliferation and differentiation rate of bone marrow cells.
辐射防护剂通过中和自由基的作用，减少辐射的破坏作用。在本方案文章中，使用微核试验评估了维生素A对γ射线诱导的微核的辐射防护作用。在2格雷（Gy）γ射线照射前两小时，以100和400毫克/千克的剂量腹腔注射维生素A。24小时后处死动物，然后将骨髓标本涂片并染色。在多色细胞中计数微核的数量。两种维生素A剂量均降低了骨髓多色红细胞（MnPCE）中的微核水平，具有统计学意义。小鼠中用于防护的维生素A适宜剂量为100毫克/千克，可保护小鼠骨髓免受辐射的致断裂作用。研究结果表明，维生素A可能通过抗氧化机制消除电离辐射产生的自由基对骨髓细胞的影响，并减少遗传损伤。•维生素A辐射防护作用的数据表明，在2 Gyγ射线照射前给小鼠施用100毫克/千克维生素A可使PCE细胞中的微核水平降低2.62倍。•施用100毫克/千克维生素A（远小于维生素A的半数致死剂量，腹腔注射的半数致死剂量=1510±240毫克/千克）可保护小鼠。•维生素A由于其抗氧化活性和捕获辐射产生的自由基，减少了电离辐射对DNA的有害影响，并减少了辐射引起的遗传损伤。•维生素A对骨髓细胞的增殖和分化率没有影响。
