Naeeji A, Mozdarani H, Shabestani Monfared A, Faeghi F, Ahmadi A A, Gholami M, Behzadi R, Momtaz M R
Radiology Technology Department, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
J Biomed Phys Eng. 2017 Jun 1;7(2):117-126. eCollection 2017 Jun.
In many studies, chemicals and natural materials were tested to reduce the harmful effects of radiation. It is known that Famotidine and vitamin C reduce DNA damage.
The aim of this study was to evaluate the radioprotective effect of vitamin C, Cimetidine and Famotidine on gamma-radiation-induced damage on mouse bone marrow.
Six-to-seven week male NMRI mice (28 g ±3) were randomly divided into fourteen groups: control, 2Gy irradiation, six group drugs without irradition (Famotidine, Cimetidine, vitaminC, Fam-Cim, Fam-Vit, Cim-Vit), six groups received drugs and 2Gy radiation with a 60Co |γ|-ray source at room temperature 22 ± 2 °C. The mice were killed 48 hours after irradiation by cervical dislocation. Slides were prepared from bone marrow cells and stained in May-Granwald and Giemsa. Finally, the cells were counted with microscope, frequencies of polychromatic erythrocyte (PCE), normochoromatic erythrocyte (NCE) and their micronuclated cell were recorded. PCE / PCE + NCE were calculated.
There were significant differences of MNPCE/1000PCE, MNNCE/1000NCE and PCE/PCE+NCE among different groups with similar radiation doses (p≤0.01). Moreover, there were significant differences of MNPCE/1000PCE and PCE/PCE+NCE among different doses of radiation (p≤0.01). While considering MNNCE/1000NCE, there were no significant differences among silimar groups with radiation dose (p˃0.05).
Oral administration of Famotidine, vitamin C and Cimetidine demonstrate reliable and similar radioprotective effects. Additionally, the protective effect of single use of these drugs was similar to the combination form. Thus, the oral use of combination, 48 hours after irradiation cannot induce more radioprotective effect.
在许多研究中，对化学物质和天然材料进行了测试，以降低辐射的有害影响。已知法莫替丁和维生素C可减少DNA损伤。
本研究旨在评估维生素C、西咪替丁和法莫替丁对γ射线诱导的小鼠骨髓损伤的辐射防护作用。
将6至7周龄的雄性NMRI小鼠（28 g±3）随机分为14组：对照组、2 Gy照射组、6组未照射药物组（法莫替丁、西咪替丁、维生素C、法莫-西咪、法莫-维生素、西咪-维生素）、6组接受药物和2 Gy辐射组，在室温22±2°C下用60Coγ射线源照射。照射后48小时通过颈椎脱臼处死小鼠。从骨髓细胞制备玻片，并用May-Granwald和吉姆萨染色。最后，用显微镜对细胞进行计数，记录多色红细胞（PCE）、正色红细胞（NCE）及其微核化细胞的频率。计算PCE/PCE + NCE。
在相似辐射剂量的不同组之间，MNPCE/1000PCE、MNNCE/1000NCE和PCE/PCE + NCE存在显著差异（p≤0.01）。此外，在不同辐射剂量之间，MNPCE/1000PCE和PCE/PCE + NCE存在显著差异（p≤0.01）。在考虑MNNCE/1000NCE时，相似辐射剂量组之间没有显著差异（p˃0.05）。
口服法莫替丁、维生素C和西咪替丁具有可靠且相似的辐射防护作用。此外，这些药物单独使用的保护作用与联合使用形式相似。因此，照射后48小时口服联合用药不能诱导更多的辐射防护作用。
