近红外光免疫疗法对丝状伪足的改变：三维低相干定量相显微镜评估
Alterations of filopodia by near infrared photoimmunotherapy: evaluation with 3D low-coherent quantitative phase microscopy.
作者信息
Nakamura Yuko, Nagaya Tadanobu, Sato Kazuhide, Harada Toshiko, Okuyama Shuhei, Choyke Peter L, Yamauchi Toyohiko, Kobayashi Hisataka
机构信息
Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, NIH, 10 Center Dr. Bethesda,MD 20892, USA.
Central Research Laboratory, Hamamatsu Photonics K.K., 5000, Hirakuchi, Hamakita-ku, Hamamatsu 434-8601, Japan.
出版信息
Biomed Opt Express. 2016 Jun 22;7(7):2738-48. doi: 10.1364/BOE.7.002738. eCollection 2016 Jul 1.
Filopodia are highly organized cellular membrane structures that facilitate intercellular communication. Near infrared photoimmunotherapy (NIR-PIT) is a newly developed cancer treatment that causes necrotic cell death. Three-dimensional low-coherent quantitative phase microscopy (3D LC-QPM) is based on a newly established low-coherent interference microscope designed to obtain serial topographic images of the cellular membrane. Herein, we report rapid involution of filopodia after NIR-PIT using 3D LC-QPM. For 3T3/HER2 cells, the number of filopodia decreased immediately after treatment with significant differences. Volume and relative height of 3T3/HER2 cells increased immediately after NIR light exposure, but significant differences were not observed. Thus, disappearance of filopodia, evaluated by 3D LC-QPM, is an early indicator of cell membrane damage after NIR-PIT.
丝状伪足是高度有序的细胞膜结构，有助于细胞间通讯。近红外光免疫疗法（NIR-PIT）是一种新开发的癌症治疗方法，可导致坏死性细胞死亡。三维低相干定量相显微镜（3D LC-QPM）基于新建立的低相干干涉显微镜，旨在获取细胞膜的系列形貌图像。在此，我们报告使用3D LC-QPM观察到NIR-PIT后丝状伪足迅速退化。对于3T3/HER2细胞，处理后丝状伪足数量立即减少，差异显著。近红外光照射后，3T3/HER2细胞的体积和相对高度立即增加，但未观察到显著差异。因此，通过3D LC-QPM评估，丝状伪足的消失是NIR-PIT后细胞膜损伤的早期指标。
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