Schultz R J, Harvey G P, Fernandez-Beros M E, Krishnamurthy S, Rodriguez J E, Cabello F
Lasers Surg Med. 1986;6(5):445-8. doi: 10.1002/lsm.1900060505.
The effects of laser energy on three bacterial strains, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were studied utilizing the neodymium:YAG laser. Cell suspensions of each strain were divided into four groups. In group I, suspensions from each strain were exposed to laser energy densities of 555-3,333 J/cm2. In groups II and III, two artificial dyes, congo red or methylene blue, were added to the suspensions prior to lasing. In group IVa, no laser energy was used, and group IVb was used to measure the bactericidal thermal effects of the laser. It was concluded that: Low dosages of laser energy exceeding 1,667 J/cm2 resulted in a 2 to 8 log decline in the number of viable bacterial colonies in vitro. Compared to the other two bacterial strains, P aeruginosa was the most sensitive to YAG laser irradiation. Addition of methylene blue, a dark-colored dye, enhanced the bactericidal effects of the YAG laser as indicated by the significantly reduced viability of P aeruginosa after irradiation with 2,222 J/cm2.
钇铝石榴石激光研究了激光能量对三种细菌菌株，即金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的影响。每种菌株的细胞悬液被分为四组。在第一组中，每种菌株的悬液暴露于555 - 3333 J/cm²的激光能量密度下。在第二组和第三组中，在激光照射前向悬液中加入两种人工染料，刚果红或亚甲蓝。在第四组a中，不使用激光能量，第四组b用于测量激光的杀菌热效应。得出以下结论：体外，超过1667 J/cm²的低剂量激光能量导致活菌菌落数量下降2至8个对数级。与其他两种细菌菌株相比，铜绿假单胞菌对钇铝石榴石激光照射最敏感。如用2222 J/cm²照射后铜绿假单胞菌的活力显著降低所示，添加深色染料亚甲蓝增强了钇铝石榴石激光的杀菌效果。
