CIUSSS du Nord-de-l'Île-de-Montréal, Hôpital Sacré-Cœur, Montréal, Canada.
Lallemand Health Solutions, Montréal, Canada.
PLoS One. 2019 Apr 22;14(4):e0215101. doi: 10.1371/journal.pone.0215101. eCollection 2019.
Caspase-3 activation in the limbic system and depressive-like symptoms are observed after an acute myocardial infarction (MI) and studies suggest that inflammation may play a significant role. Combined treatment with the probiotic strains Bifidobacterium longum and Lactobacillus helveticus in rats has been shown to attenuate caspase-3 activation and depressive-like behaviour together with a reduction in pro-inflammatory cytokines. The present study was designed to determine the respective contribution of these two strains on caspase-3 activity in the limbic system and on depressive-like behaviour. Sprague-Dawley rats were assigned to one of four groups: Vehicle, L. helveticus R0052, B. longum R0175 and L. salivarius HA-118, administered orally for 14 days (109CFU daily) before inducing MI by occlusion of the left anterior descending artery for 40 min followed by 14 days of reperfusion. Animals were then tested for socialisation, passive avoidance and forced swim test to assess depressive-like behaviour. At day 18 the animals were sacrificed; infarct size was estimated, plasma C-reactive protein concentration and brain caspase-3 activity were measured. Results indicated that infarct size did not vary across the different treatments. Rats treated with B. longum spent more time socializing, learned more rapidly the passive avoidance test and spent less time immobile in the forced swim test compared to the vehicle groups. Caspase-3 activity and plasma C-reactive protein concentrations were reduced in the lateral and medial amygdala as well as in the dentate gyrus of B. longum-supplemented animals. The only significant effect in the two groups receiving Lactobacilli compared to vehicle was that rats receiving L. salivarius learned more rapidly in the step-down passive avoidance test. In conclusion, most of the beneficial effects that we previously reported with the combination of two probiotic strains in our experimentation regarding post-myocardial infarction depression are related to Bifidobacterium longum.
在急性心肌梗死（MI）后，观察到边缘系统中的 Caspase-3 激活和类似抑郁的症状，研究表明炎症可能起重要作用。在大鼠中联合使用双歧杆菌长双歧杆菌和乳杆菌 helveticus 的治疗已被证明可减轻 Caspase-3 激活和类似抑郁的行为，同时减少促炎细胞因子。本研究旨在确定这两种菌株对边缘系统中 Caspase-3 活性和类似抑郁行为的各自贡献。将 Sprague-Dawley 大鼠分为四组之一：载体，乳杆菌 helveticus R0052，双歧杆菌 R0175 和乳杆菌 salivarius HA-118，通过左前降支闭塞 40 分钟诱导 MI 之前，每天口服给药 14 天（每天 109CFU），然后再进行 14 天的再灌注。然后，对动物进行社交、被动回避和强迫游泳测试，以评估类似抑郁的行为。在第 18 天，处死动物；估计梗死面积，测量血浆 C-反应蛋白浓度和脑 Caspase-3 活性。结果表明，不同治疗方法之间的梗死面积没有差异。与载体组相比，用双歧杆菌治疗的大鼠在社交方面花费的时间更多，在被动回避测试中更快地学习，并且在强迫游泳测试中不动的时间更少。补充双歧杆菌的动物的外侧和内侧杏仁核以及齿状回中的 Caspase-3 活性和血浆 C-反应蛋白浓度降低。与载体相比，接受乳杆菌的两组中唯一的显著作用是接受乳杆菌唾液乳杆菌的大鼠在步下式被动回避测试中更快地学习。总之，我们之前在我们的实验中报道的关于心肌梗死后抑郁的两种益生菌联合使用的大多数有益效果都与双歧杆菌长双歧杆菌有关。
