Department of Sport Sciences, University of Birjand, Birjand, Iran.
Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Pharmacol Rep. 2019 Jun;71(3):503-508. doi: 10.1016/j.pharep.2019.02.006. Epub 2019 Feb 12.
The present study was conducted to investigate the effect of intense intermittent exercise and Ginkgo biloba on the hippocampal levels of brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4) and also memory and learning in young rats.
Forty two eight-week-old rats were randomly divided into six groups including control, low dose of Ginkgo biloba (65 mg/kg), high dose of Ginkgo biloba (100 mg/kg), exercise, exercise + low dose of Ginkgo biloba, exercise + high dose of Ginkgo biloba. The exercise protocol or Ginkgo biloba administration was six days a week for six weeks. The hippocampal levels of BDNF and NT-4 were measured by ELISA method, and learning and memory were evaluated by Morris water maze test in all groups. Data were analyzed using SPSS software.
Increase in hippocampal levels of BDNF and NT-4 appeared following exercise (p < 0.01). The levels do not change following exercise + Ginkgo biloba administration. However, the NT-4 level decreased in the high dose of Ginkgo biloba group (p < 0.01). Disorder in learning and memory was indicated following the use of low dose of Ginkgo biloba or exercise + low dose Ginkgo biloba administration (p < 0.001). Learning elevated in the exercise group (p < 0.05).
Exercise in young rats may increase brain neurotrophin levels and lead to improved learning. The preventative or protective role of Ginkgo biloba against some diseases has been suggested, but its consumption in young athletes is recommended with caution.
本研究旨在探讨剧烈间歇运动和银杏叶对年轻大鼠海马脑源性神经营养因子（BDNF）和神经营养因子-4（NT-4）水平以及记忆和学习的影响。
将 42 只 8 周龄大鼠随机分为 6 组，包括对照组、银杏叶低剂量组（65mg/kg）、银杏叶高剂量组（100mg/kg）、运动组、运动+银杏叶低剂量组、运动+银杏叶高剂量组。运动方案或银杏叶给药每周 6 天，持续 6 周。采用 ELISA 法检测海马 BDNF 和 NT-4 水平，Morris 水迷宫试验评价各组学习记忆能力。数据采用 SPSS 软件进行分析。
运动后海马 BDNF 和 NT-4 水平升高（p<0.01）。运动后银杏叶给药不能改变 BDNF 和 NT-4 水平。然而，银杏叶高剂量组 NT-4 水平降低（p<0.01）。银杏叶低剂量组或运动+低剂量银杏叶给药后学习和记忆障碍（p<0.001）。运动组学习能力提高（p<0.05）。
年轻大鼠运动可增加脑神经营养因子水平，促进学习能力提高。银杏叶具有预防或保护某些疾病的作用，但不建议年轻运动员使用。
