Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Bam University of Medical Sciences, Bam, Kerman, Iran.
Biomed Res Int. 2022 Apr 26;2022:4065118. doi: 10.1155/2022/4065118. eCollection 2022.
Neuroprotective effects of stem cells have been shown in some neurologic diseases. In this study, the effect of oral mucosal mesenchymal stem cells (OMSCs) on traumatic brain injury (TBI) was evaluated in long term.
TBI was induced by Marmarou's method. The number of 2 × 10 OMSCs was intravenously injected 1 and 24 h after the injury. Brain edema and pathological outcome were assessed at 24 h and 21 days after the injury. Besides, long-term neurological, motor, and cognitive outcomes were evaluated at days 3, 7, 14, and 21 after the injury.
OMSCs administration could significantly inhibit microglia proliferation, and reduce brain edema and neuronal damage, at 24 h and 21 days after the injury. Neurological function improvement was observed in the times evaluated in OMSCs group. Cognitive and motor function dysfunction and anxiety-like behavior were prevented especially at 14 and 21 days after the injury in the treatment group.
According to the results of this study, OMSCs administration after TBI reduced brain edema and neuronal damage, improved neurologic outcome, and prevented memory and motor impairments and anxiety-like behavior in long term.
干细胞具有神经保护作用，已在一些神经疾病中得到证实。在这项研究中，我们评估了口腔黏膜间充质干细胞（OMSCs）对创伤性脑损伤（TBI）的长期影响。
采用 Marmarou 法诱导 TBI。损伤后 1 天和 24 小时，经静脉注射 2×10 OMSCs。损伤后 24 小时和 21 天评估脑水肿和病理结果。此外，在损伤后 3、7、14 和 21 天评估长期神经、运动和认知结果。
损伤后 24 小时和 21 天，给予 OMSCs 治疗可显著抑制小胶质细胞增殖，减轻脑水肿和神经元损伤。在评估的时间点，神经功能均有改善。在治疗组中，尤其是在损伤后 14 天和 21 天，认知和运动功能障碍以及焦虑样行为得到预防。
根据这项研究的结果，TBI 后给予 OMSCs 治疗可减轻脑水肿和神经元损伤，改善神经功能，并可预防长期的记忆和运动障碍以及焦虑样行为。
