College of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100000, China.
Genes (Basel). 2019 Apr 21;10(4):311. doi: 10.3390/genes10040311.
Aquaporins (AQPs), which are members of the major intrinsic protein (MIP) family, play an important role in the transport of water and other small, uncharged solutes across membranes. In this study, we identified gene encoding two aquaporin 12-like (AQP12L) proteins, and , from , a serious rice pest in Asia. Phylogenetic analysis indicated that AQP12L_V1 and AQP12L_V2 were grouped in a well-supported cluster that included other members of Lepidoptera. The two proteins are almost identical, except that AQP12L_V1 lacks 34 amino acids that are present in AQP12L_V2 at site 217. The qRT-PCR indicated that both and were expressed in heads, epidermis, foregut, midgut, and hindguts, with the highest level of expression in hindguts, heads, and epidermis. Expression of and was detected in all life stages and both sexes and was highest in first instar larvae and lowest in eggs. Expression of and was not significantly altered by exposure to brief changes in temperature. There were no significant differences in the third instar larvae, male and female pupae, and female adults in response to adverse humidity. However, the mRNA level of in the fifth instar larvae and in male adults was induced significantly by low humidity, respectively. Moreover, oocytes injected with cRNAs of AQP12L_V1 and AQP12L_V2 showed no significant changes in permeability to water, glycerol, trehalose, or urea. The two AQP12L variants likely localize to an intracellular location in and may respond to novel stimuli.
水通道蛋白（AQP）是主要内在蛋白（MIP）家族的成员，在跨膜运输水和其他小的、不带电荷的溶质方面发挥重要作用。在这项研究中，我们从亚洲严重的水稻害虫 中鉴定出编码两个水通道蛋白 12 样（AQP12L）蛋白的基因 和 。系统发育分析表明，AQP12L_V1 和 AQP12L_V2 被分组在一个支持度很高的簇中，该簇包括鳞翅目其他成员。这两种蛋白质几乎完全相同，只是 AQP12L_V1 在 217 位缺失了 34 个氨基酸，而 AQP12L_V2 存在这些氨基酸。qRT-PCR 表明 和 在头部、表皮、前肠、中肠和后肠中均有表达，在后肠、头部和表皮中的表达水平最高。 和 在所有生活阶段和雌雄个体中均有表达，在 1 龄幼虫和卵中表达水平最低。短暂温度变化暴露后， 和 的表达没有明显改变。在 3 龄幼虫、雌雄蛹和雌成虫中，对不利湿度的反应没有显著差异。然而，在 5 龄幼虫中 和在雄成虫中 的 mRNA 水平分别因低湿度而显著诱导。此外，注射了 AQP12L_V1 和 AQP12L_V2 的 cRNA 的卵母细胞对水、甘油、海藻糖或尿素的通透性没有明显变化。这两种 AQP12L 变体可能定位于 和 的细胞内位置，并且可能对新的刺激做出反应。
