Yin Chuanlin, Liu Ying, Liu Jinding, Xiao Huamei, Huang Shuiqing, Lin Yongjun, Han Zhaojun, Li Fei
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The key laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, Jiangsu 210095, China, Department of Computer Science, College of Information Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China and National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China.
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The key laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, Jiangsu 210095, China, Department of Computer Science, College of Information Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China and National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/The key laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, Jiangsu 210095, China, Department of Computer Science, College of Information Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China and National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China.
Database (Oxford). 2014 Jul 4;2014. doi: 10.1093/database/bau065. Print 2014.
ChiloDB is an integrated resource that will be of use to the rice stem borer research community. The rice striped stem borer (SSB), Chilo suppressalis Walker, is a major rice pest that causes severe yield losses in most rice-producing countries. A draft genome of this insect is available. The aims of ChiloDB are (i) to store recently acquired genomic sequence and transcriptome data and integrate them with protein-coding genes, microRNAs, piwi-interacting RNAs (piRNAs) and RNA sequencing (RNA-Seq) data and (ii) to provide comprehensive search tools and downloadable data sets for comparative genomics and gene annotation of this important rice pest. ChiloDB contains the first version of the official SSB gene set, comprising 80,479 scaffolds and 10 221 annotated protein-coding genes. Additionally, 262 SSB microRNA genes predicted from a small RNA library, 82 639 piRNAs identified using the piRNApredictor software, 37,040 transcripts from a midgut transcriptome and 69 977 transcripts from a mixed sample have all been integrated into ChiloDB. ChiloDB was constructed using a data structure that is compatible with data resources, which will be incorporated into the database in the future. This resource will serve as a long-term and open-access database for research on the biology, evolution and pest control of SSB. To the best of our knowledge, ChiloDB is one of the first genomic and transcriptome database for rice insect pests. Database URL: http://ento.njau.edu.cn/ChiloDB.
二化螟数据库（ChiloDB）是一个综合资源库，将对二化螟研究群体有用。水稻二化螟（SSB），即二化螟（Chilo suppressalis Walker），是一种主要的水稻害虫，在大多数水稻生产国都会造成严重的产量损失。这种昆虫的基因组草图已可获取。ChiloDB的目标是：（i）存储最近获得的基因组序列和转录组数据，并将它们与蛋白质编码基因、微小RNA、与piwi相互作用的RNA（piRNA）和RNA测序（RNA-Seq）数据整合；（ii）提供全面的搜索工具和可下载数据集，用于这种重要水稻害虫的比较基因组学和基因注释。ChiloDB包含官方SSB基因集的第一个版本，由80479个支架和10221个注释的蛋白质编码基因组成。此外，从小RNA文库预测的262个SSB微小RNA基因、使用piRNA预测软件鉴定的82639个piRNA、来自中肠转录组的37040个转录本以及来自混合样本的69977个转录本都已整合到ChiloDB中。ChiloDB是使用与数据资源兼容的数据结构构建的，未来这些数据资源将被纳入该数据库。这个资源将作为一个长期开放获取的数据库，用于二化螟生物学、进化和害虫防治的研究。据我们所知，ChiloDB是首批针对水稻害虫的基因组和转录组数据库之一。数据库网址：http://ento.njau.edu.cn/ChiloDB 。
