Cinici Emine, Dilekmen Nilay, Kutlu Zerrin, Dincer Busra, Cinici Ozkan, Balta Hilal, Calık Ilknur
a Department of Ophthalmology, Faculty of Medicine, Ataturk University , Erzurum , Turkey.
b Department of Ophthalmology, Palandoken State Hospital , Erzurum , Turkey.
Cutan Ocul Toxicol. 2019 Sep;38(3):290-293. doi: 10.1080/15569527.2019.1608229. Epub 2019 May 16.
Carvone (CVN) is a natural monoterpene found in essential oils of many aromatic plant species. In this study, we investigated the protective effect of CVN against paclitaxel (PTX)-induced retinal and optic nerve cytotoxicity in rats. Twenty-four male adult Wistar albino rats (250-400 g) were randomized into four equal groups comprising six animals in each. Group 1 (control group) received intraperitoneal (i.p.) saline solution (0.5 mL/200 g) weekly for 4 weeks. Group 2 received i.p. CVN [(S)-(+)- CVN, (5S)-5-Isopropenyl-2-methyl-2-cyclohexen-1-one, CH, 25 mg/kg], while Group 3 received i.p. PTX (5 mg/kg) weekly for 4 weeks. Group 4 received i.p. CVN (25 mg/kg) 30 min after i.p. PTX (5 mg/kg) weekly for 4 weeks. At the end of the experimental period, retinal and optic nerve tissues were evaluated histopathologically. All retinal specimens in control and CVN groups were histopathologically normal. In PTX group all eyes (6/6) demonstrated increased retinal vascularity and rosette-like structures in the outer nuclear layer, while in PTX-CVN group all eyes (6/6) demonstrated normal retinal vascularity and absence of rosette-like structures. All optic nerve specimens in control and CVN groups were histopathologically normal. In PTX group all eyes (6/6) demonstrated severe vacuolization and decrease in the number of astrocytes and oligodendrocytes, while 3 eyes (3/6) demonstrated marked single cell necrosis. In PTX-CVN group, 4 eyes (4/6) demonstrated moderate vacuolization while, 2 eyes (2/6) had none. Compared with PTX group, 1 eye (1/6) in PTX-CVN group demonstrated a decrease in numbers of astrocytes and oligodendrocytes while 5 eyes (5/6) were normal. No remarkable single cell necrosis was observed in PTX-CVN group. Our histopathological findings demonstrated the potential protective role of CVN against PTX-induced retinal and optic nerve cytotoxicity. CVN might be a promising molecule in counteracting oxidative stress-based cytotoxicity in the field of retinal and optic nerve disorders.
香芹酮（CVN）是一种天然单萜，存在于许多芳香植物物种的精油中。在本研究中，我们调查了CVN对紫杉醇（PTX）诱导的大鼠视网膜和视神经细胞毒性的保护作用。将24只成年雄性Wistar白化大鼠（250 - 400克）随机分为四组，每组六只。第1组（对照组）每周腹腔注射（i.p.）生理盐水（0.5毫升/200克），共4周。第2组腹腔注射CVN [（S）-（+）-CVN，（5S）-5-异丙烯基-2-甲基-2-环己烯-1-酮，CH，25毫克/千克]，而第3组每周腹腔注射PTX（5毫克/千克），共4周。第4组在腹腔注射PTX（5毫克/千克）30分钟后腹腔注射CVN（25毫克/千克），每周一次，共4周。实验期结束时，对视网膜和视神经组织进行组织病理学评估。对照组和CVN组的所有视网膜标本在组织病理学上均正常。PTX组所有眼睛（6/6）均表现出视网膜血管增多以及外核层出现玫瑰花结样结构，而PTX - CVN组所有眼睛（6/6）均表现出正常的视网膜血管且无玫瑰花结样结构。对照组和CVN组的所有视神经标本在组织病理学上均正常。PTX组所有眼睛（6/6）均表现出严重空泡化以及星形胶质细胞和少突胶质细胞数量减少，而3只眼睛（3/6）表现出明显的单细胞坏死。在PTX - CVN组中，4只眼睛（4/6）表现出中度空泡化，而2只眼睛（2/6）无空泡化。与PTX组相比，PTX - CVN组1只眼睛（1/6）的星形胶质细胞和少突胶质细胞数量减少，而5只眼睛（5/6）正常。PTX - CVN组未观察到明显的单细胞坏死。我们的组织病理学结果表明CVN对PTX诱导的视网膜和视神经细胞毒性具有潜在的保护作用。在视网膜和视神经疾病领域，CVN可能是一种有前景的分子，可对抗基于氧化应激的细胞毒性。
