Department of Food Science, Sun Moon University, Natural Science 118, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si 336-708, Korea.
Next-Generation Semiconductor Technology Center, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si 336-708, Korea.
Molecules. 2022 Aug 3;27(15):4940. doi: 10.3390/molecules27154940.
In this work, the suppression of tyrosinase-related genes, including an improvement in UV absorption effects of bioconverted CS extracts (BCS), was investigated to improve the skin-whitening effect. Total polyphenols and total flavonoids, which are bioactive components, increased 2.6- and 5.4-times in bioconversion using SM4, respectively, as compared to ultrasound-assisted extracts (UCS). The effect of BCS on radical scavenging activity, UV-A absorption, and tyrosinase activity inhibition, contributing to skin-whitening, were 1.3-, 1.2-, and 1.2-times higher than those of UCS, respectively. The main component identified in high-performance liquid chromatography (HPLC) was gallic acid in both UCS and BCS, which increased by 2.9-times following bioconversion. The gene expression of tyrosinase-related proteins, including and genes, was studied to confirm the suppression of melanin synthesis by BCS in order to identify the skin-whitening mechanism, and BCS decreased both genes' expression by 1.7- and 1.6-times, demonstrating that BCS effectively suppressed melanin synthesis. These findings imply that the chestnut inner shell can be employed as a cosmetic material by simultaneously inhibiting melanogenesis and enhancing UV-A absorption through bioconversion using SM4.
在这项工作中，研究了抑制酪氨酸酶相关基因，包括改善生物转化 CS 提取物（BCS）的紫外线吸收效果，以提高皮肤美白效果。与超声辅助提取（UCS）相比，使用 SM4 进行生物转化时，总多酚和总类黄酮这两种生物活性成分分别增加了 2.6 倍和 5.4 倍。BCS 在自由基清除活性、UV-A 吸收和抑制酪氨酸酶活性方面的效果分别比 UCS 高 1.3 倍、1.2 倍和 1.2 倍，有助于皮肤美白。高效液相色谱（HPLC）鉴定的主要成分在 UCS 和 BCS 中均为没食子酸，生物转化后增加了 2.9 倍。研究了酪氨酸酶相关蛋白的基因表达，包括 和 基因，以证实 BCS 通过生物转化抑制黑色素合成的美白机制，BCS 使这两个基因的表达分别降低了 1.7 倍和 1.6 倍，表明 BCS 有效地抑制了黑色素的合成。这些发现表明，通过使用 SM4 进行生物转化，同时抑制黑色素生成和增强 UV-A 吸收，栗壳可以用作化妆品材料。
