a Agriculture Division of DowDuPont™ , Corteva Agriscience™ , Mogi Mirim , SP , Brazil.
b Agriculture Division of DowDuPont™ , Corteva Agriscience™ , Johnston , IA , USA.
GM Crops Food. 2019;10(1):35-43. doi: 10.1080/21645698.2019.1604038. Epub 2019 Apr 22.
Transgene product expression levels are measured in genetically engineered (GE) crops containing single transformation events and the measured expression levels are then utilized in food, feed, and environmental safety assessments as part of the requirements for de-regulation of the event. Many countries also require measurement of expression levels and safety assessments for GE breeding stacks, even though the breeding stacks are composed of single events that have been previously assessed. Transgene product expression levels were measured in tissues of maize, soybean, and cotton breeding stacks and each of their component single events. Expression levels in the breeding stacks were plotted against expression levels in the single events to quantify the ability of the single events to predict transgene product expression levels in the breeding stacks. These results indicate that transgene product expression levels in single events are a reliable indicator of expression levels in breeding stacks. Based on these results it is concluded that safety assessments for breeding stacks can be conducted using transgene product expression levels from single events.
转基因产物表达水平在含有单一转化事件的基因工程（GE）作物中进行测量，然后作为去调控事件要求的一部分，用于食品、饲料和环境安全评估中。许多国家还要求对基因工程育种种群进行表达水平的测量和安全性评估，即使这些育种种群是由以前已经评估过的单一事件组成的。在玉米、大豆和棉花育种种群及其各自的单一事件的组织中测量了转基因产物的表达水平。将育种种群中的表达水平与单一事件中的表达水平进行比较，以量化单一事件预测育种种群中转基因产物表达水平的能力。这些结果表明，单一事件中转基因产物的表达水平是育种种群中表达水平的可靠指标。基于这些结果，可以得出结论，使用单一事件中转基因产物的表达水平可以对育种种群进行安全性评估。
