Que Qiudeng, Chilton Mary-Dell M, de Fontes Cheryl M, He Chengkun, Nuccio Michael, Zhu Tong, Wu Yuexuan, Chen Jeng S, Shi Liang
Syngenta Biotechnology, Inc., Research Triangle Park, NC, USA.
GM Crops. 2010 Jul-Sep;1(4):220-9. doi: 10.4161/gmcr.1.4.13439.
In recent years, there has been a rapid increase in the planting of transgenic crops with stacked traits. Most of these products have been formed by conventional breeding, i.e. the crossing of transgenic plant (event) containing individual transgenes with other event(s) containing single or double transgenic traits. Many biotech companies are developing stacked trait products with increasing numbers of insect and herbicide tolerance genes for controlling a broad range of insect pests and weeds. There has also been an increase in development of technologies for molecular stacking of multiple traits in a single transgene locus. In this review we look at the status of stacked trait products, crop trait stacking technologies and the technical challenges we are facing. We also review recent progress in developing technology for assembling large transgene arrays in vitro (molecular stacks), their delivery to crop plants and issues they pose for transgene expression.
近年来，具有叠加性状的转基因作物种植迅速增加。这些产品大多是通过传统育种形成的，即含有单个转基因的转基因植物（事件）与含有单或双转基因性状的其他事件进行杂交。许多生物技术公司正在开发具有越来越多抗虫和耐除草剂基因的叠加性状产品，以控制广泛的害虫和杂草。在单个转基因位点进行多个性状分子叠加的技术开发也有所增加。在本综述中，我们探讨了叠加性状产品的现状、作物性状叠加技术以及我们面临的技术挑战。我们还回顾了体外组装大型转基因阵列（分子叠加）技术、将其导入作物植物以及它们对转基因表达所带来问题的最新进展。
