Brassinin 通过抑制 PI3K/Akt/mTOR 信号级联来抑制肺癌细胞的侵袭潜能。
Brassinin Represses Invasive Potential of Lung Carcinoma Cells through Deactivation of PI3K/Akt/mTOR Signaling Cascade.
机构信息
KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Korea.
Department of Science in Korean Medicine, Kyung Hee University, 24 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Korea.
出版信息
Molecules. 2019 Apr 22;24(8):1584. doi: 10.3390/molecules24081584.
Abstract
The epithelial-mesenchymal transition (EMT) is a phenomenon that facilitates epithelial cells to acquire invasive potential to induce the initiation the metastatic spread of tumor cells. Here, we determined if brassinin (BSN) can affect the EMT process and deciphered its anti-cancer effects. BSN attenuated the levels of EMT linked genes and suppressed transforming growth factor beta (TGF-β)-mediated regulation of diverse mesenchymal markers. Additionally, BSN did increase the expression of various epithelial marker proteins in lung cancer cells. TGF-β-induced morphological changes and induction of invasive ability of tumor cells was also found to be abrogated by BSN treatment. Finally, BSN not only suppressed constitutive, but also inducible phosphoinositide-3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) phosphorylation in tumor cells.
摘要
上皮-间充质转化（EMT）是一种使上皮细胞获得侵袭潜能的现象，从而诱导肿瘤细胞转移扩散的起始。在这里，我们确定了藜芦硫素（BSN）是否可以影响 EMT 过程，并解析了其抗癌作用。BSN 降低了 EMT 相关基因的水平，并抑制了转化生长因子-β（TGF-β）对多种间充质标志物的调节。此外，BSN 确实增加了肺癌细胞中各种上皮标志物蛋白的表达。BSN 处理还发现可以阻止 TGF-β诱导的肿瘤细胞形态变化和侵袭能力的诱导。最后，BSN 不仅抑制了肿瘤细胞中组成性的，而且还抑制了可诱导的磷酸肌醇 3-激酶（PI3K）/蛋白激酶 B（Akt）/雷帕霉素靶蛋白（mTOR）磷酸化。
相似文献
1
Brassinin Represses Invasive Potential of Lung Carcinoma Cells through Deactivation of PI3K/Akt/mTOR Signaling Cascade.
Molecules. 2019 Apr 22;24(8):1584. doi: 10.3390/molecules24081584.
2
Brassinin induces apoptosis in PC-3 human prostate cancer cells through the suppression of PI3K/Akt/mTOR/S6K1 signaling cascades.
Phytother Res. 2014 Mar;28(3):423-31. doi: 10.1002/ptr.5010. Epub 2013 May 20.
3
Ginkgolic Acid Inhibits Invasion and Migration and TGF-β-Induced EMT of Lung Cancer Cells Through PI3K/Akt/mTOR Inactivation.
J Cell Physiol. 2017 Feb;232(2):346-354. doi: 10.1002/jcp.25426. Epub 2016 Jun 2.
4
TGF-β2 induces epithelial-mesenchymal transition in cultured human lens epithelial cells through activation of the PI3K/Akt/mTOR signaling pathway.
Mol Med Rep. 2016 Feb;13(2):1105-10. doi: 10.3892/mmr.2015.4645. Epub 2015 Dec 4.
5
Brassinin enhances the anticancer actions of paclitaxel by targeting multiple signaling pathways in colorectal cancer cells.
Phytother Res. 2021 Jul;35(7):3875-3885. doi: 10.1002/ptr.7095. Epub 2021 Apr 1.
6
Alisertib induces cell cycle arrest and autophagy and suppresses epithelial-to-mesenchymal transition involving PI3K/Akt/mTOR and sirtuin 1-mediated signaling pathways in human pancreatic cancer cells.
Drug Des Devel Ther. 2015 Jan 17;9:575-601. doi: 10.2147/DDDT.S75221. eCollection 2015.
7
Farnesol abrogates epithelial to mesenchymal transition process through regulating Akt/mTOR pathway.
Pharmacol Res. 2019 Dec;150:104504. doi: 10.1016/j.phrs.2019.104504. Epub 2019 Oct 31.
8
MiR-206 inhibits HGF-induced epithelial-mesenchymal transition and angiogenesis in non-small cell lung cancer via c-Met /PI3k/Akt/mTOR pathway.
Oncotarget. 2016 Apr 5;7(14):18247-61. doi: 10.18632/oncotarget.7570.
9
Sodium Propionate Attenuates the Lipopolysaccharide-Induced Epithelial-Mesenchymal Transition via the PI3K/Akt/mTOR Signaling Pathway.
J Agric Food Chem. 2020 Jun 17;68(24):6554-6563. doi: 10.1021/acs.jafc.0c01302. Epub 2020 Jun 8.
10
Structure Identification of ViceninII Extracted from and the Reversal of TGF-β1-Induced Epithelial⁻Mesenchymal Transition in Lung Adenocarcinoma Cells through TGF-β/Smad and PI3K/Akt/mTOR Signaling Pathways.
Molecules. 2019 Jan 2;24(1):144. doi: 10.3390/molecules24010144.
引用本文的文献
1
Kinase signaling cascades: an updated mechanistic landscape.
Chem Sci. 2025 Aug 19. doi: 10.1039/d5sc04657b.
2
Anti-metastatic potential of flavonoids for the treatment of cancers: focus on epithelial-mesenchymal transition (EMT) process.
Naunyn Schmiedebergs Arch Pharmacol. 2025 May 28. doi: 10.1007/s00210-025-04235-3.
3
To investigate the effect and mechanism of tetrahydrocurcumin on hepatocellular carcinoma based on phosphoinositide 3-kinases/AKT signaling pathway.
World J Gastrointest Oncol. 2025 Mar 15;17(3):102187. doi: 10.4251/wjgo.v17.i3.102187.
4
The Proapoptotic Effect of MB-653 Is Associated with the Modulation of Metastasis and Invasiveness-Related Signalling Pathways in Human Colorectal Cancer Cells.
Biomolecules. 2025 Jan 6;15(1):72. doi: 10.3390/biom15010072.
5
Brassinin from Brassica campestris L. inhibits colorectal cancer by inducing p62/NRF2/GPX4-regulated ferroptosis.
Animal Model Exp Med. 2025 Jul;8(7):1155-1165. doi: 10.1002/ame2.12521. Epub 2025 Jan 23.
6
Brassinin alleviates cancer cachexia by suppressing diverse inflammatory mechanisms in mice.
MedComm (2020). 2024 May 28;5(6):e558. doi: 10.1002/mco2.558. eCollection 2024 Jun.
7
Anticancer Potential of Indole Phytoalexins and Their Analogues.
Molecules. 2024 May 19;29(10):2388. doi: 10.3390/molecules29102388.
8
The Apoptotic and Anti-Warburg Effects of Brassinin in PC-3 Cells via Reactive Oxygen Species Production and the Inhibition of the c-Myc, SIRT1, and β-Catenin Signaling Axis.
Int J Mol Sci. 2023 Sep 10;24(18):13912. doi: 10.3390/ijms241813912.
9
PI3K/AKT/mTOR signaling transduction pathway and targeted therapies in cancer.
Mol Cancer. 2023 Aug 18;22(1):138. doi: 10.1186/s12943-023-01827-6.
10
Drug target therapy and emerging clinical relevance of exosomes in meningeal tumors.
Mol Cell Biochem. 2024 Jan;479(1):127-170. doi: 10.1007/s11010-023-04715-1. Epub 2023 Apr 4.
本文引用的文献
1
Ophiopogonin D, a Steroidal Glycoside Abrogates STAT3 Signaling Cascade and Exhibits Anti-Cancer Activity by Causing GSH/GSSG Imbalance in Lung Carcinoma.
Cancers (Basel). 2018 Nov 8;10(11):427. doi: 10.3390/cancers10110427.
2
Bergamottin Suppresses Metastasis of Lung Cancer Cells through Abrogation of Diverse Oncogenic Signaling Cascades and Epithelial-to-Mesenchymal Transition.
Molecules. 2018 Jul 2;23(7):1601. doi: 10.3390/molecules23071601.
3
Ophiopogonin D modulates multiple oncogenic signaling pathways, leading to suppression of proliferation and chemosensitization of human lung cancer cells.
Phytomedicine. 2018 Feb 1;40:165-175. doi: 10.1016/j.phymed.2018.01.002. Epub 2018 Jan 12.
4
Pan-HDAC inhibition by panobinostat mediates chemosensitization to carboplatin in non-small cell lung cancer via attenuation of EGFR signaling.
Cancer Lett. 2018 Mar 28;417:152-160. doi: 10.1016/j.canlet.2017.12.030. Epub 2018 Jan 4.
5
Inhibitory effect of brassinin on TNF‑α‑induced vascular inflammation in human umbilical vein endothelial cells.
Mol Med Rep. 2017 Nov;16(5):6890-6895. doi: 10.3892/mmr.2017.7406. Epub 2017 Aug 31.
6
Antiproliferative Effect of Indole Phytoalexins.
Molecules. 2016 Nov 26;21(12):1626. doi: 10.3390/molecules21121626.
7
Ascochlorin Enhances the Sensitivity of Doxorubicin Leading to the Reversal of Epithelial-to-Mesenchymal Transition in Hepatocellular Carcinoma.
Mol Cancer Ther. 2016 Dec;15(12):2966-2976. doi: 10.1158/1535-7163.MCT-16-0391. Epub 2016 Oct 7.
8
Ginkgolic Acid Inhibits Invasion and Migration and TGF-β-Induced EMT of Lung Cancer Cells Through PI3K/Akt/mTOR Inactivation.
J Cell Physiol. 2017 Feb;232(2):346-354. doi: 10.1002/jcp.25426. Epub 2016 Jun 2.
9
Exosome-Mediated Metastasis: From Epithelial-Mesenchymal Transition to Escape from Immunosurveillance.
Trends Pharmacol Sci. 2016 Jul;37(7):606-617. doi: 10.1016/j.tips.2016.04.006. Epub 2016 May 3.
10
Progression and metastasis of lung cancer.
Cancer Metastasis Rev. 2016 Mar;35(1):75-91. doi: 10.1007/s10555-016-9618-0.
Zotero 插件