Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
Department of Applied Bioscience, College of Life and Environmental Sciences, Konkuk University, Seoul 05029, Korea.
Molecules. 2019 Apr 22;24(8):1586. doi: 10.3390/molecules24081586.
A considerable amount of bioactive compounds have been used for the biopharmaceutical engineering to help human health and nutrition. Hairy root culture (HRC) or transgenic root is a favourable alternative technique for phytochemical production. is a significant source of pharmaceutically important active compounds with an enormous range of health care applications. HRC of was developed using for the production of polyphenolic compounds with antioxidant, antimicrobial, antidiabetic, anticancer and anti-inflammatory pharmaceutical activities. Hairy roots (HRs) were selected by morphological assessment, genetic and molecular analyses. The maximum accumulation of fresh mass (94.15 g/L) and dry mass (9.45 g/L) was recorded in MS liquid medium supplemented with 30 g/L sucrose at 28 days. Furthermore, HRs successfully produced numerous polyphenolic compounds, including six hydroxycinnamic acids, seven flavonols, seven hydroxybenzoic acids, vanillin, resveratrol, pyrogallol, homogentisic, and veratric acids, which were identified by UHPLC analysis. HRs produced higher total phenolic (185.65 mg/g), and flavonoid (5.25 mg/g) contents than non-transformed roots (125.55 mg/g and 3.75 mg/g). As a result of these metabolic changes, pharmaceutical activities were found higher in HRs than non-transformed roots (NTRs). The present study indicates that HRC has the potential to increase the content of beneficial polyphenolic compounds with higher potential pharmaceutical activities. To the best of our knowledge, the present study is the first report on enhancing the production of polyphenolic compounds with pharmaceutical activities from the HRCs of .
大量生物活性化合物已被用于生物制药工程，以帮助人类健康和营养。发根培养（HRC）或转基因根是植物化学物质生产的一种有利替代技术。 是具有广泛医疗保健应用的药用活性化合物的重要来源。使用 为了生产具有抗氧化、抗菌、抗糖尿病、抗癌和抗炎药物活性的多酚化合物，开发了 的 HRC。通过形态评估、遗传和分子分析选择了发根（HRs）。在补充有 30 g/L 蔗糖的 MS 液体培养基中，在第 28 天记录到最大鲜重（94.15 g/L）和干重（9.45 g/L）积累。此外，HRs 成功地产生了许多多酚化合物，包括六种羟基肉桂酸、七种类黄酮、七种羟基苯甲酸、香草醛、白藜芦醇、焦儿茶酸、原儿茶酸、和藜芦酸，这些化合物通过 UHPLC 分析得到鉴定。HRs 产生的总酚（185.65 mg/g）和类黄酮（5.25 mg/g）含量高于非转化根（125.55 mg/g 和 3.75 mg/g）。由于这些代谢变化，HRs 中的药物活性高于非转化根（NTRs）。本研究表明，HRC 具有增加有益多酚化合物含量的潜力，这些化合物具有更高的潜在药物活性。据我们所知，本研究是首次报道从 的 HRCs 中增强具有药物活性的多酚化合物的生产。
