从大鼠尿液中纯化无活性激肽释放酶。
Purification of inactive kallikrein from rat urine.
作者信息
Takaoka M, Okamura H, Iwamoto T, Morimoto S
出版信息
Adv Exp Med Biol. 1986;198 Pt A:339-45. doi: 10.1007/978-1-4684-5143-6_46.
An inactive kallikrein was purified from rat urine, and some of the properties of this enzyme were examined, in comparison with those of rat urinary kallikrein (RUK). The purified inactive kallikrein reacted with the antiserum against RUK and migrated slightly more slowly than RUK, on the immunoelectrophoresis. The molecular weights of the inactive kallikrein and RUK were estimated to be 44,000 and 38,000 by gel filtration, respectively. These results indicate that the rat urinary inactive kallikrein is immunologically identical with RUK, but this inactive enzyme has biochemical properties different from those of RUK, with respect to molecular weight and electrophoretical mobility.
从大鼠尿液中纯化出一种无活性的激肽释放酶，并与大鼠尿激肽释放酶（RUK）相比较，对该酶的一些特性进行了检测。纯化的无活性激肽释放酶与抗RUK抗血清发生反应，在免疫电泳中迁移速度比RUK略慢。通过凝胶过滤法估计，无活性激肽释放酶和RUK的分子量分别为44,000和38,000。这些结果表明，大鼠尿中的无活性激肽释放酶与RUK在免疫学上是相同的，但这种无活性酶在分子量和电泳迁移率方面具有与RUK不同的生化特性。
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