Moriya H, Ikekita M, Kizuki K
Adv Exp Med Biol. 1986;198 Pt A:35-40. doi: 10.1007/978-1-4684-5143-6_5.
Some studies on carbohydrates structure and immunological properties of human urinary kallikreins purified from the human urine of healthy men (HUK) were investigated. A general technique for fractionating asparagine-linked oligosaccharides of HUK was applied. This involves serial chromatographies on concanavalin A-Sepharose 4B and other lectins bound Agarose. Asparagine-linked main oligosaccharides of both active and inactive types of HUK were mixture of tri- or tetra-antennary complex oligosaccharide having GlcNAC beta 1-4Man beta residue (bisecting GlcNAc) (type I-2), bi-antennary complex oligosaccharide having core Fuc alpha 1-6GlcNAc residue (type III-3), tri- or tetra-antennary complex oligosaccharides having Gal beta 1-4GlcNAc beta 1-4 (Gal beta 1-4GlcNAc beta 1-2)Man alpha-residue (type I-1-A-a) etc. Besides these structural studies, the method to probe the heterogeneous profiles of HUK due to the different structures of carbohydrate chains bound, was devised without the complete purification of urinary kallikrein even with individual subject of not only active but also inactive forms of the kallikrein.
对从健康男性尿液（HUK）中纯化得到的人尿激肽释放酶的碳水化合物结构和免疫特性进行了一些研究。应用了一种分离HUK天冬酰胺连接寡糖的通用技术。这涉及在伴刀豆球蛋白A - 琼脂糖4B和其他结合琼脂糖的凝集素上进行系列色谱分析。活性和非活性类型的HUK的天冬酰胺连接主要寡糖是具有GlcNACβ1 - 4Manβ残基（平分型GlcNAc）的三分支或四分支复合寡糖（I - 2型）、具有核心Fucα1 - 6GlcNAc残基的双分支复合寡糖（III - 3型）、具有Galβ1 - 4GlcNAcβ1 - 4（Galβ1 - 4GlcNAcβ1 - 2）Manα残基的三分支或四分支复合寡糖（I - 1 - A - a型）等的混合物。除了这些结构研究外，还设计了一种方法，即使在不完全纯化尿激肽释放酶的情况下，也能探究由于结合的碳水化合物链结构不同而导致的HUK的异质性谱，不仅适用于激肽释放酶的活性形式，也适用于非活性形式的个体受试者。
