a Institute of Animal Science and Veterinary Medicine , Hubei Academy of Agricultural Sciences , Wuhan , China.
b Hubei Key Lab for Animal Embryo Engineering and Molecular Breeding , Wuhan , China.
Cell Cycle. 2019 May;18(9):1033-1044. doi: 10.1080/15384101.2019.1610241. Epub 2019 May 1.
In our previous work, gene PPP1R11 (protein phosphatase 1 regulatory subunit 11) was significantly expressed in pigs after Streptococcus suis 2 (SS2) challenged. This study firstly confirmed that SS2 induced significant expression of PPP1R11 gene in porcine alveolar macrophage (PAM) cells, and apoptosis of PAM cells were observed. After that, the core promoter of porcine PPP1R11 was identified and its transcription factor AREB6 which significantly regulated PPP1R11. We also characterized that the PPP1R11 gene is a target of miR-34a. Further, we found that PPP1R11 helped to inhibit apoptosis of PAM cells under SS2 infecting, through transcription factor AREB6 was negatively correlated with apoptosis whereas miR-34a was positively correlated. Those findings provide a functional connection among the transcription factor AREB6, miR-34a, PPP1R11 gene and apoptosis of PAM cells in the pathogenesis of the SS2 infection.
在我们之前的工作中，基因 PPP1R11（蛋白磷酸酶 1 调节亚基 11）在猪受到链球菌 2（SS2）挑战后显著表达。本研究首先证实 SS2 诱导猪肺泡巨噬细胞（PAM）细胞中 PPP1R11 基因的显著表达，并观察到 PAM 细胞凋亡。之后，鉴定了猪 PPP1R11 的核心启动子及其显著调节 PPP1R11 的转录因子 AREB6。我们还表征了 PPP1R11 基因是 miR-34a 的靶标。此外，我们发现 PPP1R11 通过转录因子 AREB6 负相关与凋亡而有助于抑制 SS2 感染时 PAM 细胞的凋亡，而 miR-34a 则呈正相关。这些发现为 SS2 感染发病机制中 AREB6、miR-34a、PPP1R11 基因和 PAM 细胞凋亡之间的转录因子 AREB6、miR-34a、PPP1R11 基因和 PAM 细胞凋亡之间的功能联系提供了证据。
