Petersson Jennifer, Jokelainen Pikka, Lassen Brian, Tagel Maarja, Viltrop Arvo, Novobilský Adam
Department of Biomedical Sciences and Veterinary Public Health, Section for Parasitology, Swedish University of Agricultural Sciences (SLU), 75007 Uppsala, Sweden.
Department of Basic Veterinary Sciences and Population Medicine, Institute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, Tartu, Estonia; Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland; Statens Serum Institut, Copenhagen, Denmark.
Vet Parasitol Reg Stud Reports. 2017 Dec;10:90-94. doi: 10.1016/j.vprsr.2017.08.013. Epub 2017 Aug 23.
Fasciolosis, an infectious disease caused by the liver fluke Fasciola hepatica, affects grazing cattle world-wide. Liver fluke F. hepatica is prevalent and well-documented in cattle in many European countries, but for the Baltic countries such information is limited. This study investigated the seroprevalence and distribution of F. hepatica in cattle in Estonia. A total of 2461 individual serum samples from 218 farms distributed throughout all 15 Estonian counties, collected between February 2012 and March 2013, were tested for specific anti-F. hepatica antibodies using an in-house enzyme-linked immunosorbent assay (ELISA). In total, 144 individual animals tested seropositive, yielding an animal-level seroprevalence of 5.9% (95% CI 5.0-6.9). The herd-level seroprevalence was 28.4% (95% CI 22.8-34.7) and the herds with at least one seropositive animal were located in 13 of the 15 counties. Of the 62 F. hepatica-positive herds, 14 (6.4%) had an in-herd seroprevalence higher than 25%. With respect to production type, the herd-level seroprevalence was 20.2%, 35.6%, and 36.4% in dairy, mixed, and beef herds, respectively. Animals from the two large islands had higher odds of testing F. hepatica-seropositive than animals from the mainland. Animals from mixed and beef herds had higher odds of testing F. hepatica-seropositive than animals from dairy herds. Mixed and beef herds, and herds with more than 100 cattle, had higher odds of having at least one seropositive animal. This study provided the first serological evidence of the presence and distribution of F. hepatica in cattle herds in Estonia.
肝片吸虫病是一种由肝片吸虫（Fasciola hepatica）引起的传染病，影响着全球范围内的放牧牛群。肝片吸虫在许多欧洲国家的牛群中普遍存在且有充分记录，但在波罗的海国家，此类信息有限。本研究调查了爱沙尼亚牛群中肝片吸虫的血清阳性率及其分布情况。在2012年2月至2013年3月期间，从爱沙尼亚全部15个县的218个农场采集了总共2461份个体血清样本，使用内部酶联免疫吸附测定（ELISA）检测其中针对肝片吸虫的特异性抗体。共有144只个体动物检测为血清阳性，动物水平的血清阳性率为5.9%（95%置信区间5.0 - 6.9）。畜群水平的血清阳性率为28.4%（95%置信区间22.8 - 34.7），至少有一只血清阳性动物的畜群分布在15个县中的13个县。在62个肝片吸虫阳性畜群中，14个（6.4%）畜群内血清阳性率高于25%。就生产类型而言，奶牛场、混合养殖场和肉牛场的畜群水平血清阳性率分别为20.2%、35.6%和36.4%。来自两个大岛的动物检测肝片吸虫血清阳性的几率高于来自大陆的动物。来自混合养殖场和肉牛场的动物检测肝片吸虫血清阳性的几率高于来自奶牛场的动物。混合养殖场和肉牛场以及拥有100头以上牛的畜群至少有一只血清阳性动物的几率更高。本研究提供了爱沙尼亚牛群中肝片吸虫存在及其分布的首个血清学证据。
