Baroudi Djamel, Khelef Djamel, Hakem Ahcene, Abdelaziz Abdelhafidh, Chen Xiaohua, Lysen Colleen, Roellig Dawn, Xiao Lihua
École Nationale Supérieure Vétérinaire, Rue Issaad Abbes, El Alia, Alger, Algeria; Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA.
École Nationale Supérieure Vétérinaire, Rue Issaad Abbes, El Alia, Alger, Algeria.
Vet Parasitol Reg Stud Reports. 2017 May;8:66-69. doi: 10.1016/j.vprsr.2017.02.005. Epub 2017 Feb 12.
Little is known on the identity and public health potential of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in farm animals in Algeria. In this study, 102 fecal specimens from pre-weaned dairy calves with or without diarrhea were collected from 19 dairy farms located in 6 provinces. PCR-restriction fragment length polymorphism analysis of the small subunit rRNA gene was used to detect and differentiate Cryptosporidium spp., whereas PCR-sequence analysis of the triosephosphate isomerase gene and ribosomal internal transcribed spacer were used to detect and genotype G. duodenalis and E. bieneusi, respectively. Cryptosporidium was found in 14 specimens, among which 7 had C. parvum, 4 had C. bovis, and 3 had mixed infection of C. parvum and C. bovis or C. bovis and C. andersoni. Subtyping of C. parvum by PCR-sequence analysis of the 60kDa glycoprotein gene identified two zoonotic subtypes IIaA16G2R1 and IIaA17G3R1. G. duodenalis was found in 28 specimens, with 6 having the host-specific assemblage E, 14 having the zoonotic assemblage A (all belonging to A2 subtype), and 8 having mixed assemblages. Six known genotypes of E. bieneusi belonging to Group 2, including I, J, BEB3, BEB4, BEB6 and PtEb XI, were identified in 11 specimens. Diarrhea was mostly associated with the occurrence of C. parvum. Data from this study suggest that human-pathogenic C. parvum subtypes and G. duodenalis and E. bieneusi genotypes are common on dairy farms in Algeria.
关于阿尔及利亚农场动物中隐孢子虫属、十二指肠贾第虫和微小隐孢子虫的身份及公共卫生潜力，人们所知甚少。在本研究中，从位于6个省份的19个奶牛场收集了102份来自断奶前腹泻或未腹泻的奶牛犊粪便样本。采用小亚基核糖体RNA基因的PCR-限制性片段长度多态性分析来检测和区分隐孢子虫属，而分别采用磷酸丙糖异构酶基因和核糖体内部转录间隔区的PCR序列分析来检测十二指肠贾第虫和微小隐孢子虫并进行基因分型。在14份样本中发现了隐孢子虫，其中7份有微小隐孢子虫，4份有牛隐孢子虫，3份有微小隐孢子虫与牛隐孢子虫或牛隐孢子虫与安氏隐孢子虫的混合感染。通过对60kDa糖蛋白基因进行PCR序列分析对微小隐孢子虫进行亚型分型，鉴定出两个人畜共患亚型IIaA16G2R1和IIaA17G3R1。在28份样本中发现了十二指肠贾第虫，其中6份具有宿主特异性组合E，14份具有人畜共患组合A（均属于A2亚型），8份具有混合组合。在11份样本中鉴定出了6种属于第2组的已知微小隐孢子虫基因型，包括I、J、BEB3、BEB4、BEB6和PtEb XI。腹泻大多与微小隐孢子虫的出现有关。本研究数据表明，在阿尔及利亚的奶牛场中，具有人致病性的微小隐孢子虫亚型以及十二指肠贾第虫和微小隐孢子虫基因型很常见。
