Maxamhud Sadiya, Reghaissia Nassiba, Laatamna AbdElKarim, Gentekaki Eleni, Tsaousis Anastasios D
Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, Kent, UK.
Laboratory of Sciences and Living Techniques, Institute of Agronomic and Veterinary Sciences, University of Souk Ahras, Annaba Road 41000, Souk Ahras, Algeria.
Parasitology. 2025 Jan;152(1):51-60. doi: 10.1017/S0031182024001616.
Gastrointestinal infections constitute a significant global health concern, particularly in tropical and subtropical regions, caused by various pathogens. Among these, spp. and are noteworthy due to their zoonotic potential. In Algeria, molecular epidemiological data on cryptosporidiosis and giardiasis are limited. To fill this gap, the present study aimed to examine the transmission dynamics of spp., and in various households. A total of 216 samples were collected from the rural Guelma and Souk Ahras provinces, located in the eastern part of Algeria. These included human and animal faeces, as well as water and soil samples. DNA was extracted, followed by nested PCR targeting the rRNA gene to detect spp., while the gene was amplified for subtyping. Detection of was performed by qPCR targeting the rRNA gene, followed by amplification of , and genes for genotyping and subtyping. Several species, including and , were identified in human, animal and environmental samples. The zoonotic subtype IIaA17G2R1 was detected in human, animal and soil samples. assemblage B was detected in a human sample, while assemblage E was found in cattle and sheep. The current investigation underscores the importance of the One Health approach in addressing issues related to intestinal parasites, highlighting the need for improved surveillance and control measures in rural settings.
胃肠道感染是一个重大的全球健康问题，尤其是在热带和亚热带地区，由多种病原体引起。其中，由于其具有人畜共患病潜力，[具体物种1]和[具体物种2]值得关注。在阿尔及利亚，关于隐孢子虫病和贾第虫病的分子流行病学数据有限。为填补这一空白，本研究旨在调查[具体物种1]、[具体物种2]和[具体物种3]在不同家庭中的传播动态。共从位于阿尔及利亚东部的农村盖勒马省和苏克阿赫拉斯省采集了216份样本。这些样本包括人类和动物粪便，以及水和土壤样本。提取DNA，然后进行巢式PCR，靶向[具体基因1]的rRNA基因以检测[具体物种1]，同时扩增[具体基因2]进行亚型分析。通过靶向[具体基因3]的rRNA基因的qPCR检测[具体物种3]，随后扩增[具体基因4]、[具体基因5]和[具体基因6]进行基因分型和亚型分析。在人类、动物和环境样本中鉴定出了几种[具体物种]，包括[具体物种1]和[具体物种2]。在人类、动物和土壤样本中检测到了人畜共患的[具体亚型]亚型IIaA17G2R1。在一份人类样本中检测到了B群，而在牛和羊中发现了E群。当前的调查强调了“同一健康（One Health）”方法在解决与肠道寄生虫相关问题方面的重要性，突出了在农村地区改善监测和控制措施的必要性。
