Dhandapani K, Sreekumar C, Sangaran A, Porteen K
Mobile Veterinary Dispensary, Salem District, Yercaud 636 601, India.
Postgraduate Research Institute in Animal Sciences, Kattupakkam, Kancheepuram District 603 203, India.
Vet Parasitol Reg Stud Reports. 2017 Jan;7:36-39. doi: 10.1016/j.vprsr.2016.12.004. Epub 2016 Dec 14.
Attempts were made to detect Neospora caninum in rats (Rattus rattus) in and around Chennai, India. During the study, 112 feral rats were trapped and blood, brain, heart, lungs and diaphragm samples were collected for serological, parasitological and molecular identification of N. caninum. Out of 112 rats, cyst-like structures were identified in 16 brain squash samples. However, the identity of the cysts could not be confirmed as N. caninum. A total of 12 sera samples were positive for N. caninum by indirect fluorescence antibody test (IFAT). Four of the 'cyst' positive samples were also positive by IFAT. None of the above samples showed amplification of N. caninum (Nc5) or toxoplasmatiid (ITS-1) fragments by PCR. In conclusion, the present study showed 10.71% seroprevalence of N. caninum among feral rats, which is a first report in India. Low prevalence of the organism in the environment and the consequent low chance of exposure of rats to N. caninum might explain the failure to detect the DNA in any of the samples tested in the study.
研究人员试图在印度钦奈及其周边地区的褐家鼠（Rattus rattus）体内检测犬新孢子虫。在研究过程中，捕获了112只野生大鼠，并采集了血液、大脑、心脏、肺和膈肌样本，用于犬新孢子虫的血清学、寄生虫学和分子鉴定。在112只大鼠中，16份脑压片样本中发现了囊样结构。然而，这些囊肿的身份无法确认为犬新孢子虫。通过间接荧光抗体试验（IFAT），共有12份血清样本对犬新孢子虫呈阳性。4份“囊肿”阳性样本通过IFAT也呈阳性。上述样本均未通过PCR扩增出犬新孢子虫（Nc5）或弓形虫（ITS-1）片段。总之，本研究显示野生大鼠中犬新孢子虫的血清阳性率为10.71%，这在印度尚属首次报道。该生物体在环境中的低流行率以及大鼠接触犬新孢子虫的机会相应较低，可能解释了在本研究中所检测的任何样本中均未检测到DNA的原因。
