Laboratório de Doenças Parasitárias - LADOPAR, Departamento de Medicina Veterinária Preventiva - DMVP, Centro de Ciências Rurais - CCR, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS, Brasil.
Rev Bras Parasitol Vet. 2022 Sep 2;31(3):e009322. doi: 10.1590/S1984-29612022049. eCollection 2022.
The seroprevalence of Sarcocystis spp. and Toxoplasma gondii was researched in swine raised in Santa Maria, RS, Brazil. Serum samples from 84 pigs from 31 farms were tested using indirect immunofluorescence assay (IFA) for both agents. Additionally, 53 samples of pork sausages and tissues destined for human consumption, including: salami, sausage, black pudding, heart, tongue, brain, and rib muscle, were submitted to PCR to detect DNA for each agent. The frequency of anti-Sarcocystis spp. antibodies was 36.9% (31/84), with titers ranging from 32 to 1024, and 25% (21/84) for anti-T. gondii antibodies, with titers ranging from 64 to 2048. Sarcocystis spp. and T. gondii DNA were detected in 67.9% (36/53) and 13.2% (7/53) of samples, respectively. The presence of antibodies and the detection of DNA from Sarcocystis spp., and T. gondii suggests that the pigs were infected and may serve as an important reservoir for both parasites. The infection by these protozoa in the swine population is relevant to public health due to their zoonotic potential.
本研究旨在调查巴西圣玛丽亚州（RS）养猪场中猪群的肉孢子虫属和刚地弓形虫血清流行率。使用间接免疫荧光试验（IFA）对来自 31 个农场的 84 份猪血清样本进行了这两种病原体的检测。此外，还对 53 份用于人类消费的猪肉香肠和组织样本（包括萨拉米香肠、香肠、血肠、心脏、舌头、大脑和肋骨肌肉）进行了 PCR 检测，以检测每种病原体的 DNA。抗肉孢子虫属抗体的阳性率为 36.9%（31/84），滴度范围为 32 至 1024；抗刚地弓形虫抗体的阳性率为 25%（21/84），滴度范围为 64 至 2048。在 67.9%（36/53）和 13.2%（7/53）的样本中分别检测到了肉孢子虫属和刚地弓形虫的 DNA。抗体的存在和肉孢子虫属及刚地弓形虫的 DNA 检测结果表明，这些猪受到了感染，可能成为这两种寄生虫的重要储存宿主。由于这些原生动物具有潜在的人畜共患性，因此它们在猪群中的感染与公共卫生相关。
