Abdullah Shadan Hassan
Department of Microbiology, College of Veterinary Medicine, Sulaimani University, Sulaimaniyah, Iraq.
Vet World. 2021 Feb;14(2):468-474. doi: 10.14202/vetworld.2021.468-474. Epub 2021 Feb 22.
Sarcocystosis is a zoonotic infection caused by various species of organisms with a worldwide geographic distribution. This study investigated the presence of organisms in cattle and sheep slaughtered at an abattoir in Sulaimani Province in North Iraq.
A total of 130 muscle samples were collected during May, June, and July of 2020, including 80 samples from sheep and 50 samples from cattle. Samples were examined visually for macrosarcocysts. The peptic digestion method was used to analyze fresh muscle tissue samples for detecting microsarcocysts followed by microscopic examination. Furthermore, muscle samples were fixed and stained with hematoxylin and eosin for histopathological examination.
In the gross examination, macroscopic cysts were not detected in both cattle and sheep; hence, all the prevalence data were obtained through microscopic observation of muscle samples. The peptic digestion method revealed the presence of banana-shaped bradyzoites in 90% and 92.5% of slaughtered cattle and sheep muscle samples, respectively. Organ-wise prevalence revealed that 95% and 92% of esophageal samples of sheep and cattle contained spp., respectively Moreover, 90% and 88% of sheep and cattle diaphragms were respectively infected. Histopathological examination of tissue sections revealed two morphologically distinct types of microsarcocysts, including thin-walled and thick-walled, in both sheep and cattle.
The suspected spp. were and in sheep and and or in cattle. Infective stages of different spp. are widespread in the study area environment.
肉孢子虫病是由多种生物引起的一种人畜共患感染病，在全球范围内均有分布。本研究调查了伊拉克北部苏莱曼尼亚省一家屠宰场宰杀的牛羊体内是否存在此类生物。
2020年5月、6月和7月共采集了130份肌肉样本，其中80份来自绵羊，50份来自牛。对样本进行肉眼检查以寻找大肉孢子囊。采用胃蛋白酶消化法分析新鲜肌肉组织样本以检测小肉孢子囊，随后进行显微镜检查。此外，将肌肉样本固定并用苏木精和伊红染色进行组织病理学检查。
在大体检查中，牛羊体内均未检测到肉眼可见的囊肿；因此，所有患病率数据均通过对肌肉样本的显微镜观察获得。胃蛋白酶消化法显示，分别有90%和92.5%的宰杀牛和羊的肌肉样本中存在香蕉形缓殖子。按器官统计的患病率显示，绵羊和牛的食管样本中分别有95%和92%含有该种生物。此外，绵羊和牛的膈肌分别有90%和88%受到感染。组织切片的组织病理学检查显示，绵羊和牛体内均有两种形态不同的小肉孢子囊，包括薄壁和厚壁的。
绵羊体内疑似的该种生物为[具体物种1]和[具体物种2]，牛体内为[具体物种3]和[具体物种4]或[具体物种5]。不同物种的感染阶段在研究区域环境中广泛存在。
