Klier J, Cabral J M, Cooney C L
Appl Biochem Biotechnol. 1986 Dec;13(3):181-7. doi: 10.1007/BF02798456.
Pseudomonas AM1 utilizes glycine and methanol to produce L-serine aerobically. The consumption of methanol in this bioconversion is stoichiometrically in excess of L-serine production. Consequently, the oxygen requirement associated with L-serine production is higher than expected for the conversion from glycine. One method of L-serine production investigated was a technique utilizing a hollow-fiber ultrafiltration cartridge as a bioreactor. Oxygen diffusion limitations appear to impede the consumption of methanol and, consequently, the production of L-serine in such a reactor. Methanol consumption data agree with predictions based on a hollow-fiber diffusion model.
假单胞菌AM1在有氧条件下利用甘氨酸和甲醇生产L-丝氨酸。在这种生物转化过程中，甲醇的消耗量在化学计量上超过了L-丝氨酸的产量。因此，与L-丝氨酸生产相关的需氧量高于从甘氨酸转化时的预期值。所研究的一种L-丝氨酸生产方法是利用中空纤维超滤柱作为生物反应器的技术。氧气扩散限制似乎阻碍了甲醇的消耗，进而阻碍了这种反应器中L-丝氨酸的生产。甲醇消耗数据与基于中空纤维扩散模型的预测结果相符。
