在假单胞菌AM1利用碳源化合物生长过程中甘氨酸和丝氨酸生物合成的相关方面。
Aspects of glycine and serine biosynthesis during growth of Pseudomonas AM1 on C compounds.
作者信息
Harder W, Quayle J R
出版信息
Biochem J. 1971 Mar;121(5):763-9. doi: 10.1042/bj1210763.
Abstract
- Methanol or formate can replace serine or glycine as supplements for growth on succinate of the auxotrophic mutants 20S and 82G of Pseudomonas AM1, showing that the organism can synthesize glycine and serine in net fashion from C(1) units. 2. Double mutants of Pseudomonas 20S and 82G have been prepared (20ST-1 and 82GT-1) that are unable to grow on succinate+1mm-glyoxylate, succinate+2mm-methanol or methanol alone. 3. Mutants 20ST-1 and 82GT-1 lacked serine-glyoxylate aminotransferase activity, and revertants to the phenotype of 20S and 82G regained serine-glyoxylate aminotransferase activity. A total revertant of 82GT-1 to wild-type phenotype regained activities of serine hydroxymethyltransferase and serine-glyoxylate aminotransferase. 4. The activity of serine-glyoxylate aminotransferase in methanol-grown Pseudomonas AM1 is eightfold higher than in the succinate-grown organism. 5. The combined results show that in Pseudomonas AM1 serine-glyoxylate aminotransferase is necessary for growth on C(1) compounds and is involved in the conversion of methanol into glycine via glyoxylate. 6. It is suggested that the phosphorylated pathway of serine biosynthesis from phosphoglycerate replenishes the supply of alpha-amino groups necessary for the flow of glyoxylate through the main assimilatory pathway during growth on C(1) compounds.
摘要
- 甲醇或甲酸可以替代丝氨酸或甘氨酸，作为嗜营养突变体铜绿假单胞菌AM1的20S和82G在琥珀酸盐上生长的补充物，这表明该生物体可以从C(1)单位以净方式合成甘氨酸和丝氨酸。2. 已制备出铜绿假单胞菌20S和82G的双突变体（20ST - 1和82GT - 1），它们无法在琥珀酸盐 + 1mM - 乙醛酸、琥珀酸盐 + 2mM - 甲醇或单独的甲醇上生长。3. 突变体20ST - 1和82GT - 1缺乏丝氨酸 - 乙醛酸转氨酶活性，回复到20S和82G表型的回复突变体恢复了丝氨酸 - 乙醛酸转氨酶活性。82GT - 1回复到野生型表型的完全回复突变体重获了丝氨酸羟甲基转移酶和丝氨酸 - 乙醛酸转氨酶的活性。4. 在以甲醇生长的铜绿假单胞菌AM1中，丝氨酸 - 乙醛酸转氨酶的活性比以琥珀酸盐生长的生物体高八倍。5. 综合结果表明，在铜绿假单胞菌AM1中，丝氨酸 - 乙醛酸转氨酶对于在C(1)化合物上生长是必需的，并且参与通过乙醛酸将甲醇转化为甘氨酸的过程。6. 有人提出，由磷酸甘油酸合成丝氨酸的磷酸化途径补充了在C(1)化合物上生长期间乙醛酸通过主要同化途径流动所需的α - 氨基供应。
相似文献
1
Aspects of glycine and serine biosynthesis during growth of Pseudomonas AM1 on C compounds.在假单胞菌AM1利用碳源化合物生长过程中甘氨酸和丝氨酸生物合成的相关方面。
Biochem J. 1971 Mar;121(5):763-9. doi: 10.1042/bj1210763.
2
The biosynthesis of serine and glycine in Pseudomonas AM1 with special reference to growth on carbon sources other than C1 compounds.假单胞菌AM1中丝氨酸和甘氨酸的生物合成，特别涉及以除C1化合物以外的碳源生长的情况。
Biochem J. 1971 Mar;121(5):753-62. doi: 10.1042/bj1210753.
3
Pathways leading to and from serine during growth of Pseudomonas AM1 on C1 compounds or succinate.在假单胞菌AM1利用C1化合物或琥珀酸盐生长期间，丝氨酸的合成与代谢途径。
Biochem J. 1970 Apr;117(3):563-72. doi: 10.1042/bj1170563.
4
Glycine formation during growth of Pseudomonas AM1 on methanol and succinate.假单胞菌AM1在甲醇和琥珀酸盐上生长过程中的甘氨酸形成。
Biochem J. 1972 Aug;128(5):1203-11. doi: 10.1042/bj1281203.
5
Microbial metabolism of C 1 and C 2 compounds. The role of glyoxylate, glycollate and acetate in the growth of Pseudomonas AM1 on ethanol and on C 1 compounds.C1和C2化合物的微生物代谢。乙醛酸、乙醇酸和乙酸盐在假单胞菌AM1利用乙醇和C1化合物生长过程中的作用。
Biochem J. 1972 Jun;128(1):107-15. doi: 10.1042/bj1280107.
6
Microbial growth on oxalate by a route not involving glyoxylate carboligase.微生物通过不涉及乙醛酸羧化酶的途径在草酸盐上生长。
Biochem J. 1970 Jun;118(1):53-9. doi: 10.1042/bj1180053.
7
Genetic and physiological control of serine and glycine biosynthesis in Saccharomyces.酿酒酵母中丝氨酸和甘氨酸生物合成的遗传与生理调控
J Bacteriol. 1972 Jan;109(1):34-43. doi: 10.1128/jb.109.1.34-43.1972.
8
Metabolism of C 1 and C 2 compounds by Pseudomonas AM1: a glycerate kinase mutant and a mutant defective in glyoxylate formation from C 1 compounds.铜绿假单胞菌AM1对C1和C2化合物的代谢：一种甘油酸激酶突变体和一种在由C1化合物形成乙醛酸方面存在缺陷的突变体。
Biochem J. 1971 Dec;125(3):66P-67P. doi: 10.1042/bj1250066p.
9
Mutants of Pseudomonas AM1 that require glycollate or glyoxylate for growth on methanol or ethanol.在甲醇或乙醇上生长需要乙醇酸或乙醛酸的假单胞菌AM1突变体。
Biochem J. 1971 Oct;124(5):74P. doi: 10.1042/bj1240074p.
10
Cleavage of malyl-Coenzyme A into acetyl-Coenzyme A and glyoxylate by Pseudomonas AM1 and other C1-unit-utilizing bacteria.假单胞菌AM1及其他利用C1单位的细菌将苹果酰辅酶A裂解为乙酰辅酶A和乙醛酸。
Biochem J. 1973 Sep;136(1):89-96. doi: 10.1042/bj1360089.
引用本文的文献
1
Unravelling Formaldehyde Metabolism in Bacteria: Road towards Synthetic Methylotrophy.解析细菌中的甲醛代谢：通往合成甲基营养型的道路
Microorganisms. 2022 Jan 20;10(2):220. doi: 10.3390/microorganisms10020220.
2
A systems biology approach uncovers cellular strategies used by Methylobacterium extorquens AM1 during the switch from multi- to single-carbon growth.系统生物学方法揭示了甲基杆菌 AM1 从多碳到单碳生长转变过程中所采用的细胞策略。
PLoS One. 2010 Nov 24;5(11):e14091. doi: 10.1371/journal.pone.0014091.
3
Alternative route for glyoxylate consumption during growth on two-carbon compounds by Methylobacterium extorquens AM1.甲基杆菌 AM1 利用二碳化合物生长时，乙醛酸的另一种代谢途径。
J Bacteriol. 2010 Apr;192(7):1813-23. doi: 10.1128/JB.01166-09. Epub 2010 Jan 29.
4
Mutants of Pseudomonas AM1 that require glycollate or glyoxylate for growth on methanol or ethanol.在甲醇或乙醇上生长需要乙醇酸或乙醛酸的假单胞菌AM1突变体。
Biochem J. 1971 Oct;124(5):74P. doi: 10.1042/bj1240074p.
5
Microbial metabolism of C 1 and C 2 compounds. The involvement of glycollate in the metabolism of ethanol and of acetate by Pseudomonas AM1.C1和C2化合物的微生物代谢。乙醇酸在假单胞菌AM1对乙醇和乙酸盐代谢中的作用。
Biochem J. 1972 Jun;128(1):99-106. doi: 10.1042/bj1280099.
6
Microbial metabolism of C 1 and C 2 compounds. The role of glyoxylate, glycollate and acetate in the growth of Pseudomonas AM1 on ethanol and on C 1 compounds.C1和C2化合物的微生物代谢。乙醛酸、乙醇酸和乙酸盐在假单胞菌AM1利用乙醇和C1化合物生长过程中的作用。
Biochem J. 1972 Jun;128(1):107-15. doi: 10.1042/bj1280107.
7
[Microbial assimilation of methanol. Isolation and characterization of the yeast Candida boidinii].[甲醇的微生物同化作用。博伊丁假丝酵母的分离与鉴定]
Arch Mikrobiol. 1972;84(1):29-42.
8
Microbial metabolism of C1 and C2 compounds. The role of acetate during growth of Pseudomonas AM1 on C1 compounds, ethanol and beta-hydroxybutyrate.C1和C2化合物的微生物代谢。醋酸盐在假单胞菌AM1利用C1化合物、乙醇和β-羟基丁酸生长过程中的作用。
Biochem J. 1973 Apr;132(4):797-801. doi: 10.1042/bj1320797.
9
Microbial growth on C-1 and C2 compounds: the metabolism of acetate to glycine in Pseudomonas AM1.C-1和C2化合物上的微生物生长：假单胞菌AM1中乙酸盐向甘氨酸的代谢
Biochem J. 1972 Nov;130(1):31P. doi: 10.1042/bj1300031pa.
10
The metabolism of nitrilotriacetate by a pseudomonad.假单胞菌对次氮基三乙酸的代谢
Biochem J. 1973 Dec;136(4):1059-68. doi: 10.1042/bj1361059.
本文引用的文献
1
Microbial growth on C(1) compounds. 5. Enzyme activities in extracts of Pseudomonas AM1.C(1) 化合物上的微生物生长。5. 假单胞菌AM1提取物中的酶活性。
Biochem J. 1963 May;87(2):386-96. doi: 10.1042/bj0870386.
2
Microbial growth on C(1) compounds. 4. Carboxylation of phosphoenolpyruvate in methanol-grown Pseudomonas AM1.C(1)化合物上的微生物生长。4. 甲醇培养的假单胞菌AM1中磷酸烯醇丙酮酸的羧化作用
Biochem J. 1962 Oct;85(1):243-50. doi: 10.1042/bj0850243.
3
4
Microbial growth on C1 compounds. II. Synthesis of cell constituents by methanol- and formate-grown Pseudomonas AM 1, and methanol-grown Hyphomicrobium vulgare.C1化合物上的微生物生长。II. 甲醇和甲酸培养的假单胞菌AM 1以及甲醇培养的普通生丝微菌的细胞成分合成
Biochem J. 1961 Dec;81(3):470-80. doi: 10.1042/bj0810470.
5
ENZYMATIC PREPARATION, STRUCTURE, AND PROPERTIES OF THIAMINE PYROPHOSPHATE-ACTIVATED FORMALDEHYDE.硫胺素焦磷酸激活的甲醛的酶法制备、结构及性质
J Biol Chem. 1965 May;240:2135-41.
6
7
Microbial growth on oxalate by a route not involving glyoxylate carboligase.微生物通过不涉及乙醛酸羧化酶的途径在草酸盐上生长。
Biochem J. 1970 Jun;118(1):53-9. doi: 10.1042/bj1180053.
8
Reactions of glycine synthesis and glycine cleavage catalyzed by extracts of Arthrobacter globiformis grown on glycine.由在甘氨酸上生长的球形节杆菌提取物催化的甘氨酸合成和甘氨酸裂解反应。
Arch Biochem Biophys. 1969 Jul;132(2):359-69. doi: 10.1016/0003-9861(69)90377-4.
9
The biosynthesis of serine and glycine in Pseudomonas AM1 with special reference to growth on carbon sources other than C1 compounds.假单胞菌AM1中丝氨酸和甘氨酸的生物合成，特别涉及以除C1化合物以外的碳源生长的情况。
Biochem J. 1971 Mar;121(5):753-62. doi: 10.1042/bj1210753.
10
Pathways leading to and from serine during growth of Pseudomonas AM1 on C1 compounds or succinate.在假单胞菌AM1利用C1化合物或琥珀酸盐生长期间，丝氨酸的合成与代谢途径。
Biochem J. 1970 Apr;117(3):563-72. doi: 10.1042/bj1170563.
Zotero 插件