Hagishita T, Yoshida T, Izumi Y, Mitsunaga T
Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Nara, Japan.
Biosci Biotechnol Biochem. 1996 Oct;60(10):1604-7. doi: 10.1271/bbb.60.1604.
Resting cells of methanol-utilizing microorganisms isolated from soils were examined for L-serine production under conditions in which L-serine-degradation was suppressed. Strain MN43, a facultative methylotrophic bacterium identified as a Methylobacterium sp., was selected for further studies. Under the optimal conditions, 65 mg/ml L-serine was produced by this bacterium from 50 mg/ml glycine and 104 mg/ml methanol in 5 days, with a molar conversion ratio from glycine to L-serine of 93%. This production is the highest so far reported for microbes producing L-serine.
对从土壤中分离出的利用甲醇的微生物的静息细胞进行了检测，以研究在L-丝氨酸降解受到抑制的条件下L-丝氨酸的产生情况。选择了菌株MN43，一种被鉴定为甲基杆菌属的兼性甲基营养细菌，进行进一步研究。在最佳条件下，该细菌在5天内从50mg/ml甘氨酸和104mg/ml甲醇中产生了65mg/ml的L-丝氨酸，甘氨酸到L-丝氨酸的摩尔转化率为93%。这一产量是迄今为止报道的微生物生产L-丝氨酸的最高产量。
